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Skip to Search Results- 11Chen, Guanqun
- 9Xu, Yang
- 7Weselake, Randall J.
- 6Caldo, Kristian Mark P.
- 4Ozga, Jocelyn
- 3Holic, Roman
- 11Agricultural, Food and Nutritional Science, Department of
- 11Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 3Biological Sciences, Department of
- 3Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 1Chemistry, Department of
- 1Chemistry, Department of/Journal Articles (Chemistry)
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2015-10-27
Jayaraman, Dhileepkumar , Zhang, Yong , Radhakrishnan, Guru V , Sederoff, Heike Winter , Malbreil, Mathilde , Sekimoto, Hiroyuki , Surek, Barbara , Delaux, Pierre-Marc, Ané, Jean-Michel , Roux, Christophe , Sussman, Michael R , Dunand, Christophe , Rothfels, Carl J , Oldroyd, Giles ED , Cheema, Jitender , Wong, Gane Ka-Shu , Stevenson, Dennis W , Melkonian, Michael , Pokorny, Lisa , Morris, Richard J , Nishiyama, Tomoaki , Volkening, Jeremy D
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2018-03-03
Holic, Roman, Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Field, Catherine J., Weselake, Randall J., Chen, Guanqun
Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioactivities including anti-cancer, anti-diabetes, anti-obesity, antioxidant, and anti-inflammatory properties. Pomegranate...
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Characterization of Type-2 Diacylglycerol Acyltransferases in the Green Microalga Chromochloris zofingiensis
Download2018-12-13
Xu, Yang, Falarz, Lucas, Chen, Guanqun
Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of the acyl-CoA-dependent TAG biosynthesis and thus is a key target for manipulating oil production in microalgae. The microalga Chromochloris zofingiensis can accumulate substantial amounts of triacylglycerol (TAG) and...
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2022-06-01
Samadi, Zahra, Mehdizadeh-Allaf, Malihe, Saifi, Reza, De Groot, Christopher T., Peerhossaini, Hassan
Mixing displays a major role in the growth of photosynthesis microorganisms in active suspensions: it gives access to nutrients, improves the microorganisms’ exposure to light, and homogenizes temperature distribution in photobioreactors (PBRs). Therefore, mixing is an essential factor in the...
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Indole-3-Acetic Acid is produced by Emiliania Huxleyi Coccolith-bearing cells and triggers a physiological response in bald cells
Download2016
Bramucci, Anna R., Labeeuw, Leen, Khey, Joleen, Case, Rebecca J., de la Mata, A. Paulina, Harynuk, James, Atwal, Harjot
Indole-3-acetic acid (IAA) is an auxin produced by terrestrial plants which influences development through a variety of cellular mechanisms, such as altering cell orientation, organ development, fertility, and cell elongation. IAA is also produced by bacterial pathogens and symbionts of plants...
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Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
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1987
Owttrim, George W., Coleman, John R.
A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as...