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- 13Singer, Stacy D.
- 11Chen, Guanqun
- 8Weselake, Randall J.
- 4Jayawardhane, Kethmi N.
- 4Xu, Yang
- 2Acharya, Surya N.
A Fluorescence‐Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase ActivityDownload
Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...
GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. Ten GPAT homologues have been identified in Arabidopsis. GPATs 4–8 have been shown to be involved in the production of extracellular lipid barrier polyesters. Recently, GPAT9 was...
Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioactivities including anti-cancer, anti-diabetes, anti-obesity, antioxidant, and anti-inflammatory properties. Pomegranate...
The demand for vegetable oils is increasing at a rapid pace due to our ever-expanding population, growing global affluence, changing dietary choices, and the need for renewable plant-derived resources. However, oilseed production is negatively impacted by unpredictable environmental conditions...
Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant EnzymeDownload
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
Characterization of the diversification of phospholipid:diacylglycerol acyltransferases in the green lineageDownload
Triacylglycerols have important physiological roles in photosynthetic organisms, and are widely used as food, feed and industrial materials in our daily life. Phospholipid:diacylglycerol acyltransferase (PDAT) is the pivotal enzyme catalyzing the acyl‐CoA‐independent biosynthesis of...
Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a ‘class a’ FATTY ACID DESATURASE 3geneDownload
Background Traditional canola (Brassica napus L.; AACC, 2n = 38) cultivars yield seed oil with a relatively high proportion of α-linolenic acid (ALA; C18:3cisΔ9,12,15), which is desirable from a health perspective. Unfortunately, due to the instability of this fatty acid, elevated levels also...
Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissueDownload
Alfalfa (Medicago sativa L.) is the most widely grown perennial forage crop and is a close relative of the model diploid legume Medicago truncatula Gaertn. However, use of alfalfa leads to substantial greenhouse gas emissions and economic losses related to inefficiencies in rumen fermentation....
Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesisDownload
Alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite the high protein content, drawbacks to their use exists, including inefficient protein digestibility and energy use efficiency in the ruminant...
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seedsDownload
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....