Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis

• Author(s) / Creator(s)

  • Owttrim, George W.
  • Coleman, John R.

• A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.

• Date created

  1987

• Subjects / Keywords

  • Gene
  • Adn/ Escherichia
  • Clonacion
  • Algue
  • Cloning
  • Genetique
  • Genetics
  • Algae
  • Plant Genetics And Breeding
  • Recombinacion
  • Mutations
  • Mutacion
  • Recombination
  • Genes
  • Escherichia Coli
  • Dna/ Escherichia
  • Clonage
  • Recombinaison
  • Genetica

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3XP6VJ2W

• License

  © 1987 George W. Owttrim et al. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.