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- 21Weselake, Randall J.
- 11Chen, Guanqun
- 8Singer, Stacy D.
- 5Caldo, Kristian Mark P.
- 5Mietkiewska, Elzbieta
- 5Siloto, Rodrigo M. P.
- 4Triacylglycerol biosynthesis
- 3Linum usitatissimum
- 2Brassica napus
Acyl-CoA-binding and self-associating properties of a recombinant 13.3 kDa N-terminal fragment of diacylglycerol acyltransferase-1 from oilseed rapeDownload
Nykiforuk, Cory L., Szarka, Steve J., Laroche, André, Mosimann, Steven C., Burton, Tracy L., Thibault, Benjamin J., Boora, Parveen S., Madhavji, Milan, Foroud, Nora A., Moloney, Maurice M., Wiehler, William B., Patterson, Nii A., Weselake, Randall J., Furukawa-Stoffer, Tara L.
Background Diacylglycerol acyltransferase (DGAT, EC 184.108.40.206) catalyzes the acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol to generate triacylglycerol and CoA. The deduced amino acid sequence of cDNAs encoding DGAT1 from plants and mammals exhibit a hydrophilic N-terminal region followed...
GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. Ten GPAT homologues have been identified in Arabidopsis. GPATs 4–8 have been shown to be involved in the production of extracellular lipid barrier polyesters. Recently, GPAT9 was...
Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioactivities including anti-cancer, anti-diabetes, anti-obesity, antioxidant, and anti-inflammatory properties. Pomegranate...
Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a ‘class a’ FATTY ACID DESATURASE 3geneDownload
Background Traditional canola (Brassica napus L.; AACC, 2n = 38) cultivars yield seed oil with a relatively high proportion of α-linolenic acid (ALA; C18:3cisΔ9,12,15), which is desirable from a health perspective. Unfortunately, due to the instability of this fatty acid, elevated levels also...
Diacylglycerol acyltransferase (DGAT) activity in relation to muscle fat content and DGAT1 genotype in two different breeds of Bos taurusDownload
To circumvent the obvious waste of resources when producing good marbled beef, research is needed to clarify mechanisms which are involved in intramuscular fat storage in cattle. The possible association between the activity of diacylglycerol acyltransferase (DGAT) and muscle fat content was...
Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissueDownload
Alfalfa (Medicago sativa L.) is the most widely grown perennial forage crop and is a close relative of the model diploid legume Medicago truncatula Gaertn. However, use of alfalfa leads to substantial greenhouse gas emissions and economic losses related to inefficiencies in rumen fermentation....
Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesisDownload
Alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite the high protein content, drawbacks to their use exists, including inefficient protein digestibility and energy use efficiency in the ruminant...
Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L.) berry and the transcriptome of the mature seedDownload
Background Sea buckthorn (Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18∶2ω-6) and...
Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesisDownload
Acyl-CoA:diacylglycerol acyltransferase (EC 220.127.116.11) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
Gene coexpression clusters and putative regulatory elements underlying seed storage reserve accumulation in ArabidopsisDownload
Background In Arabidopsis, a large number of genes involved in the accumulation of seed storage reserves during seed development have been characterized, but the relationship of gene expression and regulation underlying this physiological process remains poorly understood. A more holistic view of...