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Spring 2023
Hyaline cartilage is a strong and flexible connective tissue found throughout the human body. It provides important structural and functional support for the nose, ribs, larynx, and trachea. Due to the avascular nature of the hyaline cartilage, its defects or lesions are non-regeneratable and...
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Fall 2009
Recent advances in the fundamental theories in cryobiology using thermodynamic principles have created new opportunities for innovative methodologies in cryobiology. This thesis tested the hypothesis that calculated indicators of the two-factor hypothesis of cryoinjury, depending on cellular...
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Characterization of cryobiological responses in TF-1 cells using interrupted freezing procedures
Download2014-09-22
McGann, L. E., Elliott, J. A., Ross-Rodriguez, L. U.
Cryopreservation currently is the only method for long-term preservation of cellular viability and function for uses in cellular therapies. Characterizing the cryobiological response of a cell type is essential in the approach to designing and optimizing cryopreservation protocols. For cells used...
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Computational support systems for prediction and characterization of protein crystallization outcomes
DownloadFall 2013
Analysis of protein structures may reveal their function, regulation and interactions. Almost 90% of the known protein structures were solved using X-ray crystallography; however, many more structures remain unsolved. Protein Structure Initiative (PSI) project was created to speed up structure...
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Cryopreserved amniotic membrane as transplant allograft: viability and post-transplant outcome
Download2015-09-11
Hayward, K., Holovati, J.L. , Mokoena, T., Marquez-Curtis, L.A., Perepelkin, N.M.J., Elliott, J.A.W. , Bentley, M.J., McGann, L.E., Ross-Rodriguez, L.U.
Amniotic membrane (AM) transplantation is increasingly used in ophthalmological and dermatological surgeries to promote re-epithelialization and wound healing. Biologically active cells in the epithelial and stromal layers deliver growth factors and cytokines with anti-inflammatory,...
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2010
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies...
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2012
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...
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Fall 2011
Metabolomics aims to study all small-molecule compounds (i.e. metabolites) in cells, tissues, or biofluids. These compounds provide a functional readout of the physiological, developmental, and pathological state of a biological system. The field of metabolomics has expanded rapidly over the last...
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2014
Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.
Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...