Search
Skip to Search Results-
Spring 2015
Spindle assembly pathways are conserved in many organisms. Defects in spindle assembly can result in aneuploidy, a common cause of cell death and cancer. A better understanding of spindle assembly pathways is required to fully appreciate how defects in spindle assembly manifest as aneuploidy and...
-
Spring 2013
In the newly fertilized embryo, meiotic and mitotic spindles form consecutively within the same egg cytoplasm. The order of events during this transition is controlled, in part, via the regulation of the microtubule cytoskeleton. Herein, I show that mel-43 is part of a small paralogous gene...
-
An investigation of factors that regulate cytoskeleton in the first mitotic division of the C. elegans embryo
DownloadFall 2017
Microtubule dynamics is regulated by naturally occurring microtubule-associated proteins occurring in the cellular environment as well as by natural and synthetic compounds. These compounds target the microtubule cytoskeleton and serve as anti-mitotic drugs. Therefore, microtubules continue to be...
-
Spring 2016
Meiosis in eukaryotes includes a developmentally programmed pre-meiotic G2 phase arrest before the onset of MI division mediated by inhibitory phosphorylation of Cdk1. There are two inhibitory kinases, Wee1 and Myt1. Myt1 mediated Cyclin B-Cdk1 regulation serves as a conserved mechanism for...
-
Fall 2014
Regulation of microtubule dynamics is essential for many cellular processes, including proper assembly and function of the mitotic spindle. One mechanism for temporal and spatial regulation of microtubule dynamics is provided by the kinesin-13 microtubule depolymerizing enzymes, among which MCAK...
-
Mapping and characterization of mel-43(sb41), a gene required for early embryonic viability in C. elegans
DownloadSpring 2010
A genetic screen for dominant, temperature-sensitive, maternal-effect embryonic lethal mutations identified mel-43(sb41), a gene required for early embryonic viability (Mitenko et al., 1997). Linkage mapping placed mel-43 within a small region on chromosome IV. Genetic analyses suggested that...
-
Fall 2021
The mitotic checkpoint is a failsafe mechanism for the cell to ensure correct chromosome segregation. The evolutionarily conserved kinetochore RZZ complex is an essential component of the mitotic checkpoint. Spindly interacts with the RZZ complex in a farnesylation dependent manner and acts as a...
-
The Drosophila GW protein, a posttranscriptional gene regulator that influences progression through mitosis
DownloadSpring 2010
Regulation of mRNA translation and stability can occur in cytoplasmic compartments known as mRNA processing bodies or P bodies. These compartments contain factors that function in multiple mRNA regulatory pathways and are thought to be centres for coordinating the action of these pathways. One...
-
Characterization of GBF1, Arfs and COPI at the ER-Golgi intermediate compartment and mitotic Golgi clusters
DownloadFall 2009
Protein trafficking between the endoplasmic reticulum (ER) and Golgi complex is regulated by the activity of ADP-ribosylation factors (Arfs). Arf activation by guanine nucleotide exchange factors (GEFs) leads to the recruitment of the coatomer protein COPI and vesicle formation. By using...
-
Fall 2009
One form of protein regulation is accomplished by post-translational modification (PTM). In order to test the importance one type of PTM, methylation, in chromosome segregation, we inhibited protein methylation for brief periods in G2 using the general methylation inhibitor adenosine dialdehyde...