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Identification and Characterization of mel-43 in C. elegans

  • Author / Creator
    Ataeian, Maryam
  • In the newly fertilized embryo, meiotic and mitotic spindles form consecutively within the same egg cytoplasm. The order of events during this transition is controlled, in part, via the regulation of the microtubule cytoskeleton. Herein, I show that mel-43 is part of a small paralogous gene family that is required to specify the meiosis II program of cell division in the fertilized egg. RNAi of mel-43 and paralogues resulted in failed meiosis I polar body extrusion, followed by direct entry into mitotic prophase. Furthermore, the dominant maternal-effect mutation mel- 43(sb41) showed a meiosis-to-mitosis transition delay and a failure to segregate chromatids that exhibited persistent REC-8/kleisin immunostaining. Anti-MEL-43 antibodies showed that the MEL-43 proteins exhibited high cytoplasmic levels in meiosis as well as a fibrous staining pattern in the midzone of the anaphase spindle. The protein levels were reduced after meiosis, and this was dependent on the CUL- 2/ZYG-11 E3 ligase complex.

  • Subjects / Keywords
  • Graduation date
    2013-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R39X1B
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Biological Sciences
  • Specialization
    • Molecular Biology and Genetics
  • Supervisor / co-supervisor and their department(s)
    • Srayko, Martin (Department of Biological Sciences)
  • Examining committee members and their departments
    • Campbell, Shelagh (Department of Biological Sciences)
    • Waskiewicz, Andrew (Department of Biological Sciences)
    • Srayko, Martin (Department of Biological Sciences)
    • Stuart, David (Department of Biochemistry)