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Skip to Search Results- 19Chen, Guanqun
- 18Xu, Yang
- 9Caldo, Kristian Mark P.
- 8Weselake, Randall J.
- 7Singer, Stacy D.
- 6Mietkiewska, Elzbieta
- 10DGAT
- 5Saccharomyces cerevisiae
- 5Triacylglycerol biosynthesis
- 4LACS
- 4Linum usitatissimum
- 4Triacylglycerol
- 19Agricultural, Food and Nutritional Science, Department of
- 19Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Graduate and Postdoctoral Studies (GPS), Faculty of
- 1Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
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Improving the production of punicic acid in baker’s yeast by engineering genes in acyl channeling processes and adjusting precursor supply
Download2021-01-01
Wang, Juli, Xu, Yang, Holic, Roman, Yu, Xiaochen, Singer, Stacy D., Chen, Guanqun
ABSTRACT Punicic acid (PuA) is a high-value edible conjugated fatty acid with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, feeding and oleochemical industries. Since the production of PuA is severely limited by the fact that its natural source...
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
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2020-11-01
Xu, Yang, Mietkiewska, Elzbieta, Sha, Saleh, Weselake, Randall J., Chen, Guanqun
Punicic acid (PuA; 18:3Δ9cis,11trans,13cis), a conjugated linolenic acid isomer bearing three conjugated double bonds, is associated with various health benefits and has potential for industrial use. The major nature source of this unusual fatty acid is pomegranate (Punica granatum) seed oil,...
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Characterization of the diversification of phospholipid:diacylglycerol acyltransferases in the green lineage
Download2020-06-15
Falarz, Lucas J., Xu, Yang, Caldo, Kristian Mark P., Garroway, Colin J., Singer, Stacy D., Chen, Guanqun
Triacylglycerols have important physiological roles in photosynthetic organisms, and are widely used as food, feed and industrial materials in our daily life. Phospholipid:diacylglycerol acyltransferase (PDAT) is the pivotal enzyme catalyzing the acyl‐CoA‐independent biosynthesis of...
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Physaria fendleri and Ricinus communis LCAT-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
Download2020-10-27
Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Mietkiewska, Elzbieta, Greer, Michael S., Tian, Bo, Dyer, John M., Smith, Mark, Zhou, Xue-Rong, Qiu, Xiao, Weselake, Randall J., Chen, Guanqun
Producing hydroxy fatty acids (HFAs) in transgenic crops represents a promising strategy to meet our demands for specialized plant oils with industrial applications. The expression of Ricinus communis (castor) OLEATE 12-HYDROXYLASE (RcFAH12) in Arabidopsis has resulted in only...
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The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops
Download2020-08-27
Subedi, Udaya, Jayawardhane, Kethmi N., Pan, Xue, Ozga, Jocelyn, Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
A continuous rise in demand for vegetable oils, which comprise mainly the storage lipid triacylglycerol, is fueling a surge in research efforts to increase seed oil content and improve fatty acid composition in oilseed crops. Progress in this area has been achieved using both conventional...
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A Fluorescence‐Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity
Download2019-09-02
Falarz, Lucas, Xu, Yang, Singer, Stacy D., Chen, Guanqun
Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...
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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
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Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with a-linolenic acid
Download2018-04-01
Xu, Yang, Holic, Roman, Li, Darren, Pan, Xue, Mietkiewska, Elzbieta, Chen, Guanqun, Ozga, Jocelyn, Weselake, Randall J.
Seed oil from flax (Linum usitatissimum) is enriched in a-linolenic acid (ALA; 18:3?9cis,12cis,15cis), but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic actions...