The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops

• Author(s) / Creator(s)

  • Subedi, Udaya
  • Jayawardhane, Kethmi N.
  • Pan, Xue
  • Ozga, Jocelyn
  • Chen, Guanqun
  • Foroud, Nora A.
  • Singer, Stacy D.

• A continuous rise in demand for vegetable oils, which comprise mainly the storage lipid
  triacylglycerol, is fueling a surge in research efforts to increase seed oil content and
  improve fatty acid composition in oilseed crops. Progress in this area has been achieved
  using both conventional breeding and transgenic approaches to date. However, further
  advancements using traditional breeding methods will be complicated by the polyploid
  nature of many oilseed crops and associated time constraints, while public perception and
  the prohibitive cost of regulatory processes hinders the commercialization of transgenic
  oilseed crops. As such, genome editing using CRISPR/Cas is emerging as a breakthrough
  breeding tool that could provide a platform to keep pace with escalating demand while
  potentially minimizing regulatory burden. In this review, we discuss the technology itself
  and progress that has been made thus far with respect to its use in oilseed crops to
  improve seed oil content and quality. Furthermore, we examine a number of genes that
  may provide ideal targets for genome editing in this context, as well as new CRISPRrelated
  tools that have the potential to be applied to oilseed plants and may allow
  additional gains to be made in the future.

• Date created

  2020-08-27

• Subjects / Keywords

  • CRISPR/Cas
  • oilseed quality
  • biotechnology
  • crop improvement
  • oil yield
  • prime editing

• Type of Item

  Article (Draft / Submitted)

• DOI

  https://doi.org/10.7939/r3-229w-ge72

• License

  This is the peer reviewed version of the following article: Subedi, U., Jayawardhane, K. N., Pan, X., Ozga, J., Chen, G., Foroud, N. A., & Singer, S. D. (2020). The Potential of Genome Editing for Improving Seed Oil Content and Fatty Acid Composition in Oilseed Crops. Lipids., which has been published in final form at https://doi.org/10.1002/lipd.12249. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.