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2019-09-02

Falarz, Lucas, Xu, Yang, Singer, Stacy D., Chen, Guanqun

Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...

2006

Nykiforuk, Cory L., Szarka, Steve J., Laroche, André, Mosimann, Steven C., Burton, Tracy L., Thibault, Benjamin J., Boora, Parveen S., Madhavji, Milan, Foroud, Nora A., Moloney, Maurice M., Wiehler, William B., Patterson, Nii A., Weselake, Randall J., Furukawa-Stoffer, Tara L.

Background Diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) catalyzes the acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol to generate triacylglycerol and CoA. The deduced amino acid sequence of cDNAs encoding DGAT1 from plants and mammals exhibit a hydrophilic N-terminal region followed...

2016

Jayawardhane, Kethmi, Tomasi, Pernell, Dyer, John M., Weselake, Randall J., Mietkiewska, Elzbieta, Singer, Stacy D., Chen, Guanqun

GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. Ten GPAT homologues have been identified in Arabidopsis. GPATs 4–8 have been shown to be involved in the production of extracellular lipid barrier polyesters. Recently, GPAT9 was...

2018-03-03

Holic, Roman, Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Field, Catherine J., Weselake, Randall J., Chen, Guanqun

Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioactivities including anti-cancer, anti-diabetes, anti-obesity, antioxidant, and anti-inflammatory properties. Pomegranate...

2020-03-01

Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun

Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...

2019-12-26

Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun

Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...

2020-06-15

Falarz, Lucas J., Xu, Yang, Caldo, Kristian Mark P., Garroway, Colin J., Singer, Stacy D., Chen, Guanqun

Triacylglycerols have important physiological roles in photosynthetic organisms, and are widely used as food, feed and industrial materials in our daily life. Phospholipid:diacylglycerol acyltransferase (PDAT) is the pivotal enzyme catalyzing the acyl‐CoA‐independent biosynthesis of...

2018-12-13

Xu, Yang, Falarz, Lucas, Chen, Guanqun

Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of the acyl-CoA-dependent TAG biosynthesis and thus is a key target for manipulating oil production in microalgae. The microalga Chromochloris zofingiensis can accumulate substantial amounts of triacylglycerol (TAG) and...

2020-06-13

Subedi, Udaya, Ozga, Jocelyn A., Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.

The demand for vegetable oils is increasing at a rapid pace due to our ever-expanding population, growing global affluence, changing dietary choices, and the need for renewable plant-derived resources. However, oilseed production is negatively impacted by unpredictable environmental conditions...

2022-01-01

Wang, Juli, Singer, Stacy D., Souto, Bernardo A., Asomaning, Justice, Ullah, Aman, Bressler, David, Chen, Guanqun

Abstract The expanding use of fossil fuels has caused concern in terms of both energy security and environmental issues. Therefore, attempts have been made worldwide to promote the development of renewable energy sources, among which biofuel is especially attractive. Compared to other biofuels,...