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2014-09-22

McGann, L. E., Elliott, J. A., Ross-Rodriguez, L. U.

Cryopreservation currently is the only method for long-term preservation of cellular viability and function for uses in cellular therapies. Characterizing the cryobiological response of a cell type is essential in the approach to designing and optimizing cryopreservation protocols. For cells used...

2013

Yu, H., Jomha, N. M., Elliott, J. A., Al-Abbasi, K. K., McGann, L. E.

In previous research, we successfully cryopreserved intact human articular cartilage on its bone base with high chondrocyte viability using a vitrification protocol that entailed sequential exposure to several cryopreserving agents (CPAs) at lowering temperatures resulting in a high final...

2015-09-11

Hayward, K., Holovati, J.L. , Mokoena, T., Marquez-Curtis, L.A., Perepelkin, N.M.J., Elliott, J.A.W. , Bentley, M.J., McGann, L.E., Ross-Rodriguez, L.U.

Amniotic membrane (AM) transplantation is increasingly used in ophthalmological and dermatological surgeries to promote re-epithelialization and wound healing. Biologically active cells in the epithelial and stromal layers deliver growth factors and cytokines with anti-inflammatory,...

2010

Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.

Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies...

2012

Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.

Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...

2014

Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.

Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...

2015-08-28

Prasad, V., Jomha, N.M., Forbes, J.F., McGann, L.E., Elliott, J.A.W.

2014-09-22

McGann, L. E., Ross-Rodriguez, L. U., Elliott, J. A.

There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...

2014-09-26

Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.

Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability...

2010

Forbes, J. F., Law, G. K., Weiss, A. D., Scheuerman, A., McGann, L. E., Elliott, J. A., Jomha, N. M.

Long-term biologic storage of articular cartilage has proven elusive due to cellular degradation over time or acute damage during attempts at cryopreservation. Vitrification is one option that may result in successful cryopreservation but difficulty with cryoprotective agent (CPA) toxicity at...