Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the na+/H+ exchanger

• Author(s) / Creator(s)

  • Slepkov, Emily R.
  • Rainey, Jan K.
  • Li, Xiuju J.
  • Liu, Yonghsheng S.
  • Cheng, Florence J.
  • Lindhout, Darrin A.
  • Sykes, Brian D.
  • Fliegel, Larry

• The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized the structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated to cysteine in cysteine-less NHE1. TM IV was exquisitely sensitive to mutation with 10 of 23 mutations causing greatly reduced expression and/or activity. The Phe161 → Cys mutant was inhibited by treatment with the water-soluble sulfhydryl-reactive compounds [2-(trimethylammonium)ethyl]methanethiosulfonate and [2-sulfonatoethyl]methanethiosulfonate, suggesting it is a pore-lining residue. The structure of purified TM IV peptide was determined using high resolution NMR in a CD3OH:CDCl3:H2O mixture and in Me2SO. In CD3OH: CDCl3:H2O, TM IV was structured but not as a canonical α-helix. Residues Asp159–Leu162 were a series of β-turns; residues Leu165–Pro168 showed an extended structure, and residues Ile169–Phe176 were helical in character. These three structured regions rotated quite freely with respect to the others. In Me2SO, the structure was much less defined. Our results demonstrate that TM IV is an unusually structured transmembrane segment that is exquisitely sensitive to mutagenesis and that Phe161 is a pore-lining residue.

• Date created

  2005-01-01

• Subjects / Keywords

  • Helix
  • Coupling-Constants
  • Cysteine-Scanning Mutagenesis
  • NMR
  • Membrane-Protein Structure
  • Binding-Site
  • Residues
  • Lactose Permease
  • Escherichia-Coli
  • Peptides

• Type of Item

  Article (Published)

• License

  Attribution 4.0 International