Journal Articles (Biochemistry)

2015-01-01

Li, Xiuju J., Fliegel, Larry

The SLC9A1 gene, the Na+/H+ exchanger isoform 1 is the principal plasma membrane Na+/H+ exchanger of mammalian cells and functions by exchanging one intracellular proton for one extracellular sodium. The human protein is 815 amino acids in length. Five hundred N-terminal amino acids make up the...

2001-01-01

Moor, Andrea N., Gan, Xiaohong Tracey, Karmazyn, Morris, Fliegel, Larry

The activity of the Na+/H+ exchanger has been implicated as an important contributing factor in damage to the myocardium that occurs during ischemia and reperfusion. We examined regulation of the protein in ischemic and reperfused isolated hearts and isolated ventricular myocytes. In isolated...

1995-01-01

Dyck, Jason, Silva, Norma Lucena C. L., Fliegel, Larry

We have isolated and characterized regions important for expression of the mouse NaGraphic/HGraphic exchanger gene. A 1.1-kilobase fragment upstream of the 5′-untranslated region contains specific DNA motifs characteristic of promoter and enhancer elements including a TATA box, two CAAT boxes, an...

1987-01-01

Fliegel, Larry, Ohnishi, Mmamoru, Carpenter, Michael R., Khanna, Vijay K., Reithmeier, Reinhart A. F., MacLennan, David H.

Partial amino acid sequence analysis of rabbit fast-twitch skeletal muscle calsequestrin permitted the construction of synthetic oligonucleotides that were used as both primers and probes for the synthesis and isolation of cDNAs encoding calsequestrin from neonatal rabbit skeletal muscle...

2011-01-01

Karki, Pratap, Li, Xiuju J., Schrama, David, Fliegel, Larry

The serine/threonine kinase B-Raf is the second most frequently occurring human oncogene after Ras. Mutations of B-Raf occur with the highest incidences in melanoma, and the most common mutant, V600E, renders B-Raf constitutively active. The sodium proton exchanger isoform 1 (NHE1) is a...

2002-01-01

Li, Xiuju J., Alvarez, Bernando, Casey, Joseph R., Reithmeier, Reinhart A. F., Fliegel, Larry

We examined the ability of carbonic anhydrase II to bind to and affect the transport efficiency of the NHE1 isoform of the mammalian Na+/H+ exchanger. The C-terminal region of NHE1 was expressed in Escherichia coli fused with an N-terminal glutathionineS-transferase or with a C-terminal...

2001-01-01

Guo, Lei, Lynch, Jeffrey, Nakamur, Kimitoshi, Fliegel, Larry, Kasahara, Hideko, Izumo, Seigo, Komuro, Issei, Agellon, Luis B., Michalak, Marek

Calreticulin, a Ca2+ binding chaperone of the endoplasmic reticulum, is also highly expressed in the embryonic heart, and knockout of the calreticulin gene is lethal during embryogenesis because of impaired cardiac development. The protein is down-regulated after birth, and elevated expression of...

2008-01-01

Moncoq, Karine, Kemp, Grant, Li, Xiuju J., Fliegel, Larry, Young, Howard S.

The Na+/H+ exchanger isoform 1 (NHE1) is an integral membrane protein that regulates intracellular pH by extruding an intracellular H+ in exchange for one extracellular Na+. The human NHE1 isoform is involved in heart disease and cell growth and proliferation. Although details of NHE1 regulation...

1995-01-01

Shaw, Andrew R. E., Domanska, Agatha, Mak, Allan, Gilchrist, Anita, Dobler, Kelly, Visser, Lydia, Poppema, Sibrand, Fliegel, Larry, Letarte, Michelle, Willett, Brian J.

Few molecules have been shown to confer cell motility. Although the motility-arresting properties of anti-CD9 monoclonal antibody (mAb) suggest the transmembrane 4 superfamily (TM4SF) member CD9 can induce a motorgenic signal, gene transfection studies have failed to confirm this hypothesis. We...

1990-01-01

Michalak, M., Fliegel, Larry, Wlasichuk, K.

Two major Ca2(+)-binding glycoproteins Mr 120,000 and 100,000 were isolated from 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid -solubilized bovine heart sarcolemma membrane. Peroxidase-conjugated concanavalin A and wheat germ agglutinin lectins bind strongly to the isolated 120-...