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Skip to Search Results- 6Biochemistry, Department of
- 6Biochemistry, Department of/Journal Articles (Biochemistry)
- 2Medicine and Dentistry, Faculty of
- 2Medicine and Dentistry, Faculty of/Journal Articles (Medicine and Dentistry)
- 1Agricultural, Food and Nutritional Science, Department of
- 1Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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A PagP fusion protein system for the expression of intrinsically disordered proteins in E. coli
Download2012-01-01
Hwang, Peter M., Pan, Jonathan S., Sykes, Brian D.
PagP, a beta-barrel membrane protein found in Gram-negative bacteria, expresses robustly in inclusion bodies when its signal sequence is removed. We have developed a new fusion protein expression system based on PagP and demonstrated its utility in the expression of the unstructured N-terminal...
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2007
Sawhney, Summit, Greiner, Russ, Querengesser, Lori, Shrivastava, Savita, Vogel, Hans J., Arndt, David, Macinnis, Glen D., Natalie, Zhang, Gavin E., Ying, Duggan, Tang, Peter, Amegbey, Godwin, Jewell, Kevin, Wagner, James, Clements, Tzur, Dan, Bamforth, Fiona, Young, Nelson, Miniaci, Jessica, Nikolai, Lisa, Eisner, Roman, Coutouly, Marie-Aude, Clive, Derrick, Li, Liang, Marrie, Tom, Block, David, Hau, David D., Cheng, Dean, Melisa, Gebremedhin, Stothard, Paul, Forsythe, Ian, Weljie, Alim M., Dowlatabadi, Reza, Wishart, David S., Sykes, Brian D., Fung, Chris, Knox, Craig, Jeroncic, Kevin, Mulu, Guo, Lewis, Mike, Guo, An Chi
The Human Metabolome Database (HMDB) is currently the most complete and comprehensive curated collection of human metabolite and human metabolism data in the world. It contains records for more than 2180 endogenous metabolites with information gathered from thousands of books, journal articles...
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Structural and functional analysis of transmembrane segment IV of the salt tolerance protein Sod2
Download2013-01-01
Ullah, Assad, Kemp, Grant, Lee, Brian, Alves, Claudia, Young, Howard, Sykes, Brian D., Fliegel, Larry
Sod2 is the plasma membrane Na+/H+ exchanger of the fission yeast Schizosaccharomyces pombe. It provides salt tolerance by removing excess intracellular sodium (or lithium) in exchange for protons. We examined the role of amino acid residues of transmembrane segment IV (TM IV)...
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Structural and functional analysis of transmembrane segment VI of the NHE1 isoform of the na+/H+ exchanger
Download2009-01-01
Tzeng, Jennifer, Lee, Brian L., Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein. It resides on the plasma membrane of cells and regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of...
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Structural and functional analysis of transmembrane XI of the NHE1 isoform of the na+/H+ exchanger
Download2009-01-01
Lee, Brian L., Li, Xiuju J., Liu, Yongsheng, Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of the critical transmembrane (TM) segment XI...
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Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the na+/H+ exchanger
Download2005-01-01
Slepkov, Emily R., Rainey, Jan K., Li, Xiuju J., Liu, Yonghsheng S., Cheng, Florence J., Lindhout, Darrin A., Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized the structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated to cysteine in cysteine-less NHE1. TM IV...
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Structural and functional characterization of transmembrane segment IX of the NHE1 isoform of the na(+)/H(+) exchanger
Download2008-01-01
Reddy, Tyler, Ding, Jie, Li, Xiuju J., Sykes, Brian D., Rainey, Jan K., Fliegel, Larry
The Na+/H+ exchanger isoform 1 (NHE1) is an integral membrane protein that regulates intracellular pH by removing one intracellular H+ in exchange for one extracellular Na+. It has a large N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain....
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Structural and functional characterization of transmembrane segment VII of the na+/H+ exchanger isoform 1
Download2006-01-01
Ding, Jie, Rainey, Jan K., Xu, Caroline, Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is an integral membrane protein that regulates intracellular pH by exchanging one intracellular H+ for one extracellular Na+. It is composed of an N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain. We...
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2016-01-01
Cai, Fangze, Li, Monica X., Pineda-Sanbria, Sandra E., Gelozia, Shorena, Lindert, Steffen, West, Frederick, Sykes, Brian D., Hwang, Peter M.
In cardiac and skeletal muscle, the troponin complex turns muscle contraction on and off in a calcium-dependent manner. Many small molecules are known to bind to the troponin complex to modulate its calcium binding affinity, and this may be useful in a broad range of conditions in which striated...