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- 2Cysteine-Scanning Mutagenesis
- 2Lactose Permease
- 1Activated Protein-Kinase
We examined the ability of carbonic anhydrase II to bind to and affect the transport efficiency of the NHE1 isoform of the mammalian Na+/H+ exchanger. The C-terminal region of NHE1 was expressed in Escherichia coli fused with an N-terminal glutathionineS-transferase or with a C-terminal...
Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the na+/H+ exchangerDownload
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized the structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated to cysteine in cysteine-less NHE1. TM IV...
Structural and functional characterization of transmembrane segment IX of the NHE1 isoform of the na(+)/H(+) exchangerDownload
The Na+/H+ exchanger isoform 1 (NHE1) is an integral membrane protein that regulates intracellular pH by removing one intracellular H+ in exchange for one extracellular Na+. It has a large N-terminal membrane domain of 12 transmembrane segments and an intracellular C-terminal regulatory domain....