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The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass...
Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based MetabolomicsDownload
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
Development and Application of Isotope Labeling Method and Data Processing Program for Liquid Chromatography Mass Spectrometry Based Metabolome ProfilingDownload
The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...
Development and Applications of Chemical Isotope Labeling Methods for Metabolome Sample Normalization and Liquid Chromatography-Mass Spectrometry-Based MetabolomicsDownload
Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...
Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence CharacterizationDownload
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative ProteomicsDownload
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
Development of Blood Metabolomics for Biomarker Discovery Using Chemical Isotope Labeling and Liquid Chromatography-Mass SpectrometryDownload
Blood-metabolite-based biomarkers are useful for the early-stage diagnosis, accurate prognostic prediction and personalized treatment of various diseases. Blood contains a massive number of metabolites that can potentially become biomarkers, but the metabolome coverage of current analytical...
Development of Chemical Isotope Labeling (CIL) LC-MS Techniques for Untargeted Analysis Milk Metabolomics and Targeted Analysis of Propionic AcidDownload
Metabolomics is the study of all the small molecules in a given sample or organism. It is used to understand individual variations caused by the relationship between the individual’s genetics, exposome and metabolic activity. There is interest in using metabolomics for personalized care leading...
Development of Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry for Cellular MetabolomicsDownload
Cellular metabolomics is the global study of a whole set of metabolites and its chemical processes in cells. Different analytical platforms, such as NMR and MS, have been employed for profiling the highly complex cellular metabolome and various data processing approaches have been developed. ...
Development of Data Processing Methods in Chemical Isotope Labeling Liquid Chromatography-Mass Spectrometry-Based MetabolomicsDownload
Metabolomics is an active research field on the methods development for the analysis of small metabolites in biological systems. It provides powerful approaches that allow us to examine the variations in the metabolic profiles and is capable of detecting complex biological changes using...