Chemical Isotope Labeling LC-MS for Human Saliva and Urine Metabolomics

• Author / Creator

  Tran, Tran TN

• The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass spectrometry (LC-ESI FT-ICR-MS). A method based on the use of this isotope labeling LC-MS platform was optimized for human salivary metabolome analysis, aiming for the discovery of metabolite biomarkers in mild cognitive impairment (MCI) and Alzheimer’s diseases (AD). In addition, a large scale metabolomics study was performed on 100 urine samples taken during a 3 continuous day period. All samples were analyzed using the isotope labelling liquid chromatography mass spectrometry methodology. A pragmatic approach combining well-established statistical tools was developed to process this large data set in order to generate metabolic profiles of those samples.

• Subjects / Keywords

  • Isotope Labeling
  • Metabolomics
  • Mass-spectrometry
  • LC-MS

• Graduation date

  Spring 2015

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R34746Z3H

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.