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- 4Gänzle, Michael G.
- 2Su, Marcia Shu-Wei
- 2Zhao, Xin
- 1Abdi, Reihaneh
- 1Black, Brenna A.
- 1Curtis, Jonathan M.
Characterization of α-Galactooligosaccharides formed via Heterologous Expression of α- 2 Galactosidases from Lactobacillus reuteri in Lactococcus lactisDownload
α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular...
Lactobacillus reuteri is a common organism in cereal-based foods and a gut symbiont in humans and animals, yet the molecular mechanisms allowing its persistence in various niches are not well understood. L. reuteri LTH2584 produces reutericyclin and persists in industrial sourdoughs, where acidic...
Feed fermentation formulate gut microbiota of weanling pigs - Effects of exopolysaccharides and reutericyclinDownload
Enteric bacterial infections, especially post-weaning diarrhea, are among the most common diseases affecting pigs and economically significant in swine production. Antibiotics were a cost-effective method to treat and prevent these bacterial diseases. However, with concerns of the prevalence of...
Functional characterization of sucrose phosphorylase and scrR, a regulator of sucrose metabolism in Lactobacillus reuteriDownload
Lactobacillus reuteri harbours alternative enzymes for sucrose metabolism, sucrose phosphorylase, fructansucrases, and glucansucrases. Sucrose phosphorylase and fructansucrases additionally contribute to raffinose metabolism. Glucansucrases and fructansucrases produce exopolysaccharides as...
Genomic and phenotypic comparison of Lactobacillus reuteri isolates from food and intestinal ecosystems provides insights to probiotic applicationsDownload
Some of the food fermenting lactobacilli are derived from the animal intestine. Lactobacillus reuteri provides a model organism to study molecular mechanisms of ecological adaptation from gut to food. The overall objective of this dissertation was to characterize ecological fitness of L. reuteri...
Study on metabolism of lactic acid bacteria in wheat sourdough can improve bread quality such as bread flavour, texture and shelf life. This dissertation focused on several metabolic pathways in Lactobacillus strains on both biochemical and genetic level to fulfill three objectives. The first...
Novel two-component regulatory systems play a role in biofilm formation of Lactobacillus reuteri rodent isolate 100-23Download
his study characterized the two-component regulatory systems encoded by bfrKRT and cemAKR, and assessed their influence on biofilm formation by Lactobacillus reuteri 100-23. A method for deletion of multiple genes was employed to disrupt the genetic loci of two-component systems. The operons...
Reutericyclin producing Lactobacillus reuteri modulates development of fecal microbiota in weanling pigsDownload
Lactobacillus reuteri is used as probiotic culture in food and feed applications; however, strain specific properties of L. reuteri that mediate probiotic activity remain unknown. This study aimed to determine effects of feed fermentation with exopolysaccharide and reutericyclin producing L....
Lactobacilli constitute the natural microbiota of cereal fermentations, and their competitiveness has been attributed to the formation of organic acids and various antagonistic compounds. However, these traits alone do not fully explain the prevalence of specific Lactobacillus strains in cereal...