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- 1Brennan, Lesley Jean
- 1Campbell, Stuart D.
- 1El-Yazbi, Amira F
- 1Goulko, Alevtina
- 1Lee, Megan Sae Bom
- 1Li, Xiao
Polycomb protein methyltransferase, Enhancer of Zeste 2 (Ezh2), is frequently overexpressed in human malignancy and is implicated in cancer cell proliferation and invasion. Recently, several other polycomb proteins have been implicated in modulating the response to DNA double strand breaks, and...
Development and application of a capillary electrophoresis immunoassay for DNA lesions induced by ultraviolet lightDownload
Ultraviolet (UV) light is one of the most abundant DNA damaging agents. The major DNA lesions, such as cyclobutane pyrimidine dimers (CPDs) and (6-4) pyrimidine pyrimidone (64PPs) photoproducts, are carcinogenic and mutagenic. Studies of the formation, repair and mutagenicity of these DNA lesions...
Drosophila Mage, a component of Smc5/6 DNA response complex, confers resistance to caffeine and genotoxic stress and plays a role in the cell cycle and cell survivalDownload
Over 50 Melanoma-associated antigen (MAGE) genes have been identified in the human genome. They share a conserved 200 amino acid MAGE-homology domain (MHD). In yeast, the only MAGE homolog (Nse3) is a component of the Smc5/6 DNA damage response complex. In humans, MAGE proteins influence cell...
In this thesis, we develop a sensitive, robust, accurate method to detect chemically-induced DNA damage by three therapeutic agents, cisplatin, psoralen and busulfan. The method uses fluorescence spectroscopy and fluorescent hairpin or molecular beacon probes in a hybridization assay for...
The C-terminal region of breast cancer susceptibility gene 1 (BRCA1) contains a pair of tandem BRCT repeats that are critical for the tumour suppressor function of BRCA1. BRCT repeats are present in a large of number of proteins that are implicated in the cellular response to DNA damage. A subset...
A large number of exogenous and endogenous agents can result in DNA damage leading to mutagenesis and cell death. A thorough knowledge and understanding about the effects of these agents on different sequences of DNA is important for elucidating the fundamental chemical mechanisms of DNA damage....
Absorption of UV light by nucleic acids can result in the formation of molecular lesions leading to mutagenesis, carcinogenesis, and cell death. Thus, understanding DNA damage is important for elucidating the molecular mechanisms of disease. Much effort has been focused on developing methods for...
Novel spectroscopic probes of sunscreens, initial excited-state structural dynamics and DNA photodamageDownload
This thesis discusses developing new tools to probe DNA damage resulting from photoinduced chemical processes and probing the initial excited-state structural dynamics of nucleic acids and sunscreen agents. The results of this thesis show that UV resonance Raman spectroscopy (UVRRS) is an...