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Fluorescent Hairpin Probes for the Detection of Chemically-Induced DNA Damage

  • Author / Creator
    Shire, Zahra J
  • In this thesis, we develop a sensitive, robust, accurate method to detect chemically-induced DNA damage by three therapeutic agents, cisplatin, psoralen and busulfan. The method uses fluorescence spectroscopy and fluorescent hairpin or molecular beacon probes in a hybridization assay for chemically-induced ssDNA damage probed in solution and on a microarray platform. Damage is confirmed by matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) and UV absorbance spectroscopy. The decrease in fluorescence upon damage scales with the number of mutation sites, drug doses and irradiation time. All these results indicate that the hybridization probes can quantitatively and selectively detect the number of DNA lesions/strand for chemically-induced DNA damage. Finally, fluorescent hairpin probes have some limitations, particularly with regards to their modifications and costs. However, the development of easy, fast and reliable assays is essential for biological as well as clinical applications, but requires these limitations to be addressed.

  • Subjects / Keywords
  • Graduation date
    2012-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3X05XQ0Q
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • Loppnow, Glen ( Department of Chemistry)
  • Examining committee members and their departments
    • Campbell, Robert ( Department of Chemistry)
    • Gallin, Warren ( Department of Biological Sciences)