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A role for Polycomb Repressive Complex 2 in the DNA damage response
- Author / Creator
- Campbell, Stuart D.
Polycomb protein methyltransferase, Enhancer of Zeste 2 (Ezh2), is frequently
overexpressed in human malignancy and is implicated in cancer cell proliferation and
invasion. Recently, several other polycomb proteins have been implicated in modulating
the response to DNA double strand breaks, and thus we hypothesize that Ezh2 is also
involved in the DNA damage response pathway. We investigated the involvement of Ezh2 and its associated complex, polycomb repressive complex 2 (PRC2), in the DNA damage response pathway. We found that PRC2 proteins recruit to sites of DNA damage and knockdown of Ezh2 decreases double strand break repair efficiency and increases cellular sensitivity to ionizing radiation. Our data supports the hypothesis that PRC2 is involved in the DNA damage response and contributes to DNA double strand break signaling and repair.
- Graduation date
- Spring 2013
- Type of Item
- Master of Science
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