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Spring 2015

Tran, Tran TN

The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass...

Fall 2021

Wang, Yingwen

Metabolomics refers to the characterization and quantification of small molecule metabolic products in a biological specimen. It is an emerging and evolving science studying the practice of precious medicine. Metabolomics study has been used to understand individual variations influenced by both...

Spring 2016

Huan, Tao

Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...

Spring 2015

Tseng, Chiao-Li

The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...

Fall 2015

Wu, Yiman

Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...

Fall 2013

Chen, Lu

Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...

Spring 2011

Lo, Andy

This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...

Fall 2012

Xu, Mingguo

The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...

Spring 2011

Cooper, Victoria Irene

An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10...

Spring 2018

Han, Wei

Blood-metabolite-based biomarkers are useful for the early-stage diagnosis, accurate prognostic prediction and personalized treatment of various diseases. Blood contains a massive number of metabolites that can potentially become biomarkers, but the metabolome coverage of current analytical...