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Skip to Search Results- 43Metabolomics
- 8Mass-spectrometry
- 6Chemical isotope labeling
- 5LC-MS
- 3Dansylation
- 3Metabolite identification
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...
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Metabolite Identification and Quantification in Biofluids Using Liquid Chromatography Mass Spectrometry
DownloadFall 2012
Metabolomics, one of the branches of systems biology, is the comprehensive measurement of all the endogenous metabolites in a biological system. It can be applied in the areas of biomarker discovery and diagnosis of critical diseases and disorders, and can significantly increase our understanding...
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Fall 2017
Welding is defined as the joining of metals with extreme heat, producing fumes, which consist of harmful metals and ultrafine particulates that may lead to detrimental health effects. Currently, air sampling is the primary method to determine welding fume exposure, but is not always feasible....
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Metabolomics by GC×GC-TOFMS: Overcoming Challenges from Sample Preparation to Data Analysis Towards Standardization of the Workflow
DownloadSpring 2021
Metabolomics is an ‘omics’ field, which involves the comprehensive characterization of metabolites using analytical chemistry technologies and statistical methods to interpret the results. The term metabolomics was coined about two decades ago as an analogy to other precedent omics approaches:...
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Spring 2022
Metabolomics, as an interdisciplinary research area, requires the integration of knowledge from different disciplines, such as analytical chemistry, bioinformatics, and statistics. The general workflow of metabolome analysis includes sample preparation, data acquisition, data processing, data...
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Method Development of Chemical Isotope Labeling of Cellular Extract and Biofluids on Metabolomics Profiling and Its Application for Disease Biomarker Discovery
DownloadFall 2019
Metabolomics aims at studying all the small molecules in biological samples. Compared to transcriptome and proteome, metabolome is highly sensitive to diverse individuals and environmental factors. Thus, metabolomics study has been used to understand individual variations caused by genes,...
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Nuclear Magnetic Resonance metabolomic fingerprint of the Interleukin 10 gene deficient mouse model of Inflammatory Bowel Disease
DownloadFall 2010
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder that occurs as a consequence of a genetic mutation that results in an overly aggressive immune response to normal bacteria. Metabolomics is a new born cousin to genomics and proteomics and involves a high throughput...
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Fall 2013
Cancer cachexia is a multifactorial syndrome characterized by involuntary weight loss, wasting of skeletal muscle driven by reduced food intake and abnormal metabolism. Cachexia has a negative impact on quality of life, response to chemotherapy and survival. Cachexia research is undeveloped with...
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Spring 2022
BackgroundEvidence is emerging that the gut microbiome is an important contributor to the weight and metabolic effects of bariatric surgery. However, the microbial and intestinal physiological changes that occur with bariatric surgery are poorly understood. Developing this understanding...
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Fall 2012
Pancreatic cancer is a leading cause of cancer-related death, due to its aggressive biology, lack of tools for early diagnosis and screening, advanced presentation and resistance to adjuvant therapy. Metabolomics, the newest of the “omics” sciences, may offer the potential for non-invasive...