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Skip to Search Results- 33Mass-spectrometry
- 8Metabolomics
- 4Proteomics
- 3Electrospray ionization
- 3Isotope labeling
- 3Liquid chromatography
- 1Alexander, Anthony John.
- 1Chen, Lu
- 1Dawe, Margot Renee
- 1De Souza, Andrea
- 1Dunlop, Kevin Yoshio.
- 1Fentabil, Messele
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Fall 2014
Scientists and clinicians involved in the development of biomarkers confront with the challenge of selecting the best analytical methods among dozens that have been reported in the literature. Creation of new and improvement of exist technologies is an integral part of the pathway to analytical...
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Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...
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Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
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Spring 2016
The simultaneous quantification of all biological molecules, from metabolites to proteins, holds the key to discovering molecular signatures that can diagnose diseases before irreversible damages have occurred in the body. Recent advances in the field of liquid chromatography coupled mass...
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Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2016
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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Application of Electrospray Ionization Mass Spectrometry to Study Protein-Ligand Interactions and Enzyme Kinetics
DownloadFall 2015
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) to study the noncovalent protein-ligand interactions and also investigate the kinetic of enzymatic reactions. The rate of glycolipids cleavage by the human neuraminidase 3 (hNEU3) was...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Characterization of glycosyltransferases and glycosidases using electrospray mass spectrometry
DownloadFall 2011
Carbohydrates are one of the major classes of organic compounds found in nature and living organisms, and the diverse roles of carbohydrates are crucial for most life forms. The transformation and degradation of glycans are effectively regulated by carbohydrate-modifying enzymes. This thesis...