Thin Films for Solid Matrix Laser Desorption/Ionization for Biomarker Analysis

  • Author / Creator
    Peng, Chen
  • Scientists and clinicians involved in the development of biomarkers confront with the challenge of selecting the best analytical methods among dozens that have been reported in the literature. Creation of new and improvement of exist technologies is an integral part of the pathway to analytical systems. The work of this thesis investigates new tools for biomarker analysis, which may also suggest biomarker discovery, using solid matrix assisted laser desorption/ionization mass spectrometry (SMALDI-MS) by using porous nanostructured films fabricated by the thin film process glancing angle deposition (GLAD). This project consists of three phases. The first objective of this thesis is to introduce a simple interface of DMF and GLAD, which provided an attractive proof-of-concept that GLAD is available for off-line SMALDI-MS detection. The second objective is to demonstrate a ready-to-use on-chip digestion system, which is coupled to SMALDI-MS for peptide fingerprinting mapping and is preliminary evaluated by digestion performance using a standard protein. The third objective is to investigate the performance of detecting free amino acids by modified silicon GLAD films based SMALDI. The application of GLAD nanostructured thin films to biomarker analysis SMALDI-MS is demonstrated and validated in this thesis.

  • Subjects / Keywords
  • Graduation date
    Fall 2014
  • Type of Item
  • Degree
    Doctor of Philosophy
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Mark T. McDermott (Chemistry)
    • Thomas Thundat (Chemical and Materials Engineering)
    • Robert E. Campbell (Chemistry)
    • D. Jed Harrison (Chemistry)
    • Richard Oleschuk (Chemistry)
    • Julianne M. Gibbs-Davis (Chemistry)