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1999

Owttrim, George W., Magee, Wendy C., Yu, Esther, Chamot, Danuta

The ability to modify RNA secondary structure is crucial for numerous cellular processes. We have characterized two RNA helicase genes, crhB and crhC, which are differentially expressed in the cyanobacterium Anabaena sp. strain PCC 7120. crhC transcription is limited specifically to cold shock...

2006

Colvin, Kimberley R., Owttrim, George W. , Patterson-Fortin, Laura M.

Expression of the cyanobacterial DEAD-box RNA helicase, crhR , is regulated in response to conditions, which elicit reduction of the photosynthetic electron transport chain. A combination of electrophoretic mobility shift assay (EMSA), DNA affinity chromatography and mass spectrometry identified...

2012

Stothard, Paul, Dennis, Jonathan J., Lynch, Karlene H.

Background Genomic analysis of bacteriophages infecting the Burkholderia cepacia complex (BCC) is an important preliminary step in the development of a phage therapy protocol for these opportunistic pathogens. The objective of this study was to characterize KL1 (vBBceSKL1) and AH2 (vBBceSAH2),...

2014

Owttrim, George W., Tarassova, Oxana S., Chamot, Danuta

Conditional proteolysis is a crucial process regulating the abundance of key regulatory proteins associated with the cell cycle, differentiation pathways, or cellular response to abiotic stress in eukaryotic and prokaryotic organisms. We provide evidence that conditional proteolysis is involved...

2016

Fahlman, Richard P., Owttrim, George W. , Rosana, Albert R. R., Whitford, Denise S.

The cyanobacterium Synechocystis sp. strain PCC 6803 encodes a single DEAD box RNA helicase, CrhR, whose expression is tightly autoregulated in response to cold stress. Subcellular localization and proteomic analysis results indicate that CrhR localizes to both the cytoplasmic and thylakoid...

2017-01-01

Carvalho, Wildemar S. P., Wei, Menglian, Ikpo, Nduka, Gao, Yongfeng, Serpe, Michael J.

Reports in the literature on the development of new chemical sensing technologies have been growing steadily in number for years. For example, a Web of Science topic search using the term “Chemical Sensing” revealed that over 8 000 papers were published in 2015–2017, with over 40 000 citations to...

2000

Owttrim, George W., Chamot, Danuta

Expression of the Anabaena sp. strain PCC 7120 RNA helicase gene crhC is induced by cold shock.crhC transcripts are not detectable at 30°C but accumulate at 20°C, and levels remain elevated for the duration of the cold stress. Light-derived metabolic capability, and not light per se, is required...

2006

Owttrim, George W.

RNA helicases function as molecular motors that rearrange RNA secondary structure, potentially performing roles in any cellular process involving RNA metabolism. Although RNA helicase association with a range of cellular functions is well documented, their importance in response to abiotic stress...

2009-01-01

Tzeng, Jennifer, Lee, Brian L., Sykes, Brian D., Fliegel, Larry

The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein. It resides on the plasma membrane of cells and regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of...

2009-01-01

Lee, Brian L., Li, Xiuju J., Liu, Yongsheng, Sykes, Brian D., Fliegel, Larry

The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of the critical transmembrane (TM) segment XI...