Cyanobacterial RNA Helicase CrhR Localizes to the Thylakoid Membrane Region and Cosediments with Degradosome and Polysome Complexes in Synechocystis sptrain PCC 6803

• Author(s) / Creator(s)

  • Fahlman, Richard P.
  • Owttrim, George W.
  • Rosana, Albert R. R.
  • Whitford, Denise S.

• The cyanobacterium Synechocystis sp. strain PCC 6803 encodes a single DEAD box RNA helicase, CrhR, whose expression is tightly autoregulated in response to cold stress. Subcellular localization and proteomic analysis results indicate that CrhR localizes to both the cytoplasmic and thylakoid membrane regions and cosediments with polysome and RNA degradosome components. Evidence is presented that either functional RNA helicase activity or a C-terminal localization signal was required for polysome but not thylakoid membrane localization. Polysome fractionation and runoff translation analysis results indicate that CrhR associates with actively translating polysomes. The data implicate a role for CrhR in translation or RNA degradation in the thylakoid region related to thylakoid biogenesis or stability, a role that is enhanced at low temperature. Furthermore, CrhR cosedimentation with polysome and RNA degradosome complexes links alteration of RNA secondary structure with a potential translation-RNA degradation complex in Synechocystis.

• Date created

  2016

• Subjects / Keywords

  • Cold-Shock Protein
  • Low-Temperature
  • Bacillus-Subtilis
  • Sp Pcc-6803
  • Listeria-Monocytogenes
  • Escherichia-Coli
  • Dead-Box Helicase
  • Polynucleotide Phosphorylase
  • Messenger-Rna
  • Ribosomal Maturation

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3KH0FD3H

• License

  © 2016 Albert R. R. Rosana et al. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.