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Skip to Search Results- 24Weselake, Randall J.
- 15Chen, Guanqun
- 10Singer, Stacy D.
- 7Mietkiewska, Elzbieta
- 7Xu, Yang
- 6Caldo, Kristian Mark P.
- 4DGAT
- 4Triacylglycerol
- 4Triacylglycerol biosynthesis
- 3Linum usitatissimum
- 3Saccharomyces-cerevisiae
- 2Arabidopsis thaliana
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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1999-01-01
Xie, Changping, Albrecht, Elke, Wegner, Jochen J., Brockmann, Gudrun A., Kazala, Christopher, Weselake, Randall J., Ender, Klaus
Leptin is a hormone involved in the regulation of feed intake and energy balance in animals. The expression and secretion of leptin is highly correlated with body fat mass and adipocyte size. The regulation of leptin is integrated into a broad regulatory network including other hormones and...
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Physaria fendleri and Ricinus communis LCAT-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
Download2020-10-27
Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Mietkiewska, Elzbieta, Greer, Michael S., Tian, Bo, Dyer, John M., Smith, Mark, Zhou, Xue-Rong, Qiu, Xiao, Weselake, Randall J., Chen, Guanqun
Producing hydroxy fatty acids (HFAs) in transgenic crops represents a promising strategy to meet our demands for specialized plant oils with industrial applications. The expression of Ricinus communis (castor) OLEATE 12-HYDROXYLASE (RcFAH12) in Arabidopsis has resulted in only...
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The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana
Download2021-01-01
Singer, Stacy D., Jayawardhane, Kethmi N., Jiao, Chen, Weselake, Randall J., Chen, Guanqun
ABSTRACT Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications. As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine44...
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2020-11-01
Xu, Yang, Mietkiewska, Elzbieta, Sha, Saleh, Weselake, Randall J., Chen, Guanqun
Punicic acid (PuA; 18:3Δ9cis,11trans,13cis), a conjugated linolenic acid isomer bearing three conjugated double bonds, is associated with various health benefits and has potential for industrial use. The major nature source of this unusual fatty acid is pomegranate (Punica granatum) seed oil,...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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Development and characterization of low α-linolenic acid Brassica oleracea lines bearing a novel mutation in a ‘class a’ FATTY ACID DESATURASE 3gene
Download2014
Singer, Stacy D., Weselake, Randall J., Rahman, Habibur
Background Traditional canola (Brassica napus L.; AACC, 2n = 38) cultivars yield seed oil with a relatively high proportion of α-linolenic acid (ALA; C18:3cisΔ9,12,15), which is desirable from a health perspective. Unfortunately, due to the instability of this fatty acid, elevated levels also...
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Fatty acid composition of developing sea buckthorn (Hippophae rhamnoides L.) berry and the transcriptome of the mature seed
Download2012
Snyder, Crystal L., Schroeder, William R., Wishart, David, Cram, Dustin, Fatima, Tahira, Datla, Raju, Weselake, Randall J., Krisha, Priti
Background Sea buckthorn (Hippophae rhamnoides L.) is a hardy, fruit-producing plant known historically for its medicinal and nutraceutical properties. The most recognized product of sea buckthorn is its fruit oil, composed of seed oil that is rich in essential fatty acids, linoleic (18∶2ω-6) and...