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Skip to Search Results- 13Metabolomics
- 8Mass-spectrometry
- 7LC-MS
- 6Chemical isotope labeling
- 3Metabolite identification
- 3Proteomics
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Advancing Lipidomics through Method Development, Data Processing, and its Applications in Cancer Research
DownloadFall 2024
Lipidomics, a subfield of metabolomics, offers comprehensive insights into the lipid composition of biological systems. Lipids, comprising a broad class of biological molecules, are essential to several biological functions. They play critical roles in cell membrane formation, serving as...
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Spring 2015
The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass...
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Fall 2021
Metabolomics refers to the characterization and quantification of small molecule metabolic products in a biological specimen. It is an emerging and evolving science studying the practice of precious medicine. Metabolomics study has been used to understand individual variations influenced by both...
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Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2016
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
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Development and Application of Isotope Labeling Method and Data Processing Program for Liquid Chromatography Mass Spectrometry Based Metabolome Profiling
DownloadSpring 2015
The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...
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Development and Applications of Chemical Isotope Labeling Methods for Metabolome Sample Normalization and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2015
Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...
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Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence Characterization
DownloadFall 2013
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Fall 2012
The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...
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Development of an enantioselective two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method for the analysis of methylsulfonyl polychlorinated biphenyls in tissue extracts
DownloadSpring 2011
An enantioselective heart-cut two-dimensional liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry method was developed for the analysis of 25 methylsulfonyl polychlorinated biphenyl metabolites in tissue extracts. Enantioseparation was achieved for 9 out of the 10...