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Skip to Search Results- 7Carassius-auratus
- 5Inhibition
- 4Peptides
- 3Escherichia-Coli
- 3Growth-hormone
- 3Membrane-Protein Structure
- 7Biological Sciences, Department of
- 7Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 3Biochemistry, Department of
- 3Biochemistry, Department of/Journal Articles (Biochemistry)
- 2Agricultural, Food and Nutritional Science, Department of
- 2Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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1992
Forsyth, George W., Romancyia, D. A., Uwiera, Richard R. E., Wong, Jonathan P.
The B subunit of cholera toxin has been covalently attached to the surface of Uposomes made from a mixture of phosphatidylethanolamine, phosphati- dylcholine and cholesterol. Adenylate cydase inhibitors and chloride conduc- tance inhibitors were encapsulated within the liposomes. These...
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Hypophysiotropic action of pituitary adenylate cyclase activating polypeptide (PACAP) in the goldfish: Immunohistochemical demonstration of PACAP in the pituitary, PACAP stimulation of growth hormone release from pituitary cells, and molecular cloning of goldfish pituitary type I PACAP receptor
Download1998
Shea, W.L.C., Wong, A.O.L., Chang, J.P., Tse, L.Y., Leung, M.Y., Chow, B.K.C.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a member of the glucagon/secretin peptide family, and its molecular structure is highly conserved in vertebrates. In this study, the functional role of PACAP in regulating GH release in the goldfish was investigated. Using...
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γ-Aminobutyric Acid Up-Regulates the Expression of a Novel Secretogranin-II Messenger Ribonucleic Acid in the Goldfish Pituitary
Download1998
Docherty, K., Chang, J.P., Blázquez, M., Trudeau, V.L., Bosma, P.T.
An RNA-arbitrarily primed PCR differential display strategy was used to identify candidate genes in the pituitary that are up-regulated by endogenously activated g-aminobutyric acid (GABA) systems that may also be involved in the control of reproduction. Goldfish were injected with the GABA...
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2000
Sherwood, N.M., Craig, A.G., Park, M., Powell, J.F., Carolsfeld, J., Chang, J.P., Fischer, W.H., Rivier, J.E.
The evolution of GnRH and the role of multiple forms within the brain are examined. Three forms of GnRH were purified from the brain of Pacific herring (Clupea harengus pallasi) and characterized using Edman degradation and mass spectrometry. Two forms correspond with the known structures of...
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Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the brain of a teleost, Pejerrey
Download2001
Somoza, G.M., Rivier, J.E., Fischer, W.H., Park, M.K., Chang, J.P., Craig, A.G., Montaner, A.D., Sherwood, N.M.
The neuropeptide GnRH is the major regulator of reproduction in vertebrates acting as a first signal from the hypothalamus to pituitary gonadotropes. Three GnRH molecular variants were detected in the brain of a fish, pejerrey (Odontesthes bonariensis), using chromatographic and immunological...
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Endogenous melanocortin antagonist in fish: Structure, brain mapping and regulation by fasting of the goldfish agouti-related protein gene
Download2003
Cerdá-Reverter, J.M., Peter, R.E.
Agouti-related protein (AGRP) is a naturally occurring antagonist of melanocortin. In mammals, central AGRP expression is restricted to the arcuate nucleus in which it plays a key role in the control of energy balance by antagonizing melanocortin effects at melanocortin 4 receptors. In goldfish,...
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Molecular cloning, pharmacological characterization and brain mapping of the melanocortin 4 receptor in the goldfish: Involvement in the control of food intake
Download2003
Schioth, H.B., Cerdá-Reverter, J.M., Peter, R.E., Ringholm, A.
We report cloning, pharmacological characterization, tissue distribution, detailed brain mapping, and role in control of food intake of melanocortin 4 receptor in goldfish (gMC4R). The gMC4R protein has 68% identity with the human ortholog and is conserved in important functional domains....
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Endogenous hypothalamic somatostatins differentially regulate growth hormone secretion from goldfish pituitary somatotropes in vitro
Download2003
Davis, P.J., Peter, R.E., Chang, J.P., Graves, C., Smith, S., Rivier, J.E., Yunker, W.Y., Unniappan, S.
Using Southern blot analysis of RT-PCR products, mRNA for three different somatostatin (SS) precursors (PSS-I, -II, and -III), which encode for SS14, goldfish brain (gb)SS28, and [Pro2]SS14, respectively, were detected in goldfish hypothalamus. PSS-I and -II mRNA, but not PSS-III mRNA, were also...
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Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the na+/H+ exchanger
Download2005-01-01
Slepkov, Emily R., Rainey, Jan K., Li, Xiuju J., Liu, Yonghsheng S., Cheng, Florence J., Lindhout, Darrin A., Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals. We characterized the structural and functional aspects of the critical transmembrane (TM) segment IV. Each residue was mutated to cysteine in cysteine-less NHE1. TM IV...
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Structural and functional analysis of transmembrane XI of the NHE1 isoform of the na+/H+ exchanger
Download2009-01-01
Lee, Brian L., Li, Xiuju J., Liu, Yongsheng, Sykes, Brian D., Fliegel, Larry
The Na+/H+ exchanger isoform 1 is a ubiquitously expressed integral membrane protein that regulates intracellular pH in mammals by extruding an intracellular H+ in exchange for one extracellular Na+. We characterized structural and functional aspects of the critical transmembrane (TM) segment XI...