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Skip to Search Results- 8Cryopreservation
- 6Interactions
- 5Vitrification
- 4Articular cartilage
- 3Chondrocytes
- 3Cryoprotectant agents
- 10Chemical and Materials Engineering, Department of
- 10Chemical and Materials Engineering, Department of/Research Articles and Materials (Chemical and Materials Engineering)
- 6Surgery, Department of
- 6Surgery, Department of/Orthopaedic Surgery
- 2Chemistry, Department of
- 2Chemistry, Department of/Journal Articles (Chemistry)
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Characterization of cryobiological responses in TF-1 cells using interrupted freezing procedures
Download2014-09-22
McGann, L. E., Elliott, J. A., Ross-Rodriguez, L. U.
Cryopreservation currently is the only method for long-term preservation of cellular viability and function for uses in cellular therapies. Characterizing the cryobiological response of a cell type is essential in the approach to designing and optimizing cryopreservation protocols. For cells used...
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2013
Yu, H., Jomha, N. M., Elliott, J. A., Al-Abbasi, K. K., McGann, L. E.
In previous research, we successfully cryopreserved intact human articular cartilage on its bone base with high chondrocyte viability using a vitrification protocol that entailed sequential exposure to several cryopreserving agents (CPAs) at lowering temperatures resulting in a high final...
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Cryopreserved amniotic membrane as transplant allograft: viability and post-transplant outcome
Download2015-09-11
Hayward, K., Holovati, J.L. , Mokoena, T., Marquez-Curtis, L.A., Perepelkin, N.M.J., Elliott, J.A.W. , Bentley, M.J., McGann, L.E., Ross-Rodriguez, L.U.
Amniotic membrane (AM) transplantation is increasingly used in ophthalmological and dermatological surgeries to promote re-epithelialization and wound healing. Biologically active cells in the epithelial and stromal layers deliver growth factors and cytokines with anti-inflammatory,...
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2010
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies...
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2012
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...
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2014-10-27
Wu, Xiaolei , Wafula, Eric , Yan, Zhixiang , Kutchan, Toni M. , Gitzendanner, Matthew A, Shaw, Jonathan A., Chen, Tao. , Wickett, Norman J., Nguyen, Nam , Burleigh, J Gordon , Miles, Nicholas W. , Soltis, Pamela S. , Melkonian, Michael , Deyholos, Michael K. , Matasci, Naim , DeGironimo, Lisa , Samudrala, Ram , Leebens-Mack, Jim , Mirarab, Siavash , Barker, Michael , Sun, Xiao , Soltis, Douglas E. , Rolf, Megan , Tian, Zhijian , Wong, Gane K, Rothfels, Carl , Pokorny, Lisa , Zhang, Yong , Ayyampalayam, Saravanaraj , Graham, Sean W. , Roure, Béatrice, Der, Joshua P., Wang, Jun , Mathews, Sarah , Surek, Barbara , Baucom, Regina S. , Ruhfel, Brad R. , Villarreal, Juan C., Stevenson, Dennis W. , Hung, Ling-Hong , Warnow, Tandy , Philippe, Hervé , Carpenter, Eric J.
The 1,000 plants (1KP) project is an international multi-disciplinary consortium that has generated transcriptome data from over 1,000 plant species, with exemplars for all of the major lineages across the Viridiplantae (green plants) clade. Here, we describe how to access the data used in a...
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Design and performance evaluation of vertical shafts: rational shaft design method and verification of design method
Download1988
Ground deformations around axisymmetric shafts cannot be determined with the design approaches currently available, which are mostly based on plasticity methods. The convergence–confinement method (usually applied to tunnels), with consideration of gravitational effects and the three-dimensional...
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2016
Klassen, John S., Han, Ling, Kitova, Elena N.
This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...
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2016
Han, Ling, Klassen, John S., Kitova, Elena N.
This study reports on the use of the catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay, combined with glycomicelles, as a method for detecting specific interactions between water-soluble proteins and glycolipids (GLs) in aqueous solution. The B subunit homopentamers...
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2014
Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.
Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...