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Skip to Search Results- 10Chen, Guanqun
- 9Singer, Stacy D.
- 5Xu, Yang
- 4Weselake, Randall J.
- 3Caldo, Kristian Mark P.
- 3Jayawardhane, Kethmi N.
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A Fluorescence‐Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity
Download2019-09-02
Falarz, Lucas, Xu, Yang, Singer, Stacy D., Chen, Guanqun
Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...
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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
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Characterization of the diversification of phospholipid:diacylglycerol acyltransferases in the green lineage
Download2020-06-15
Falarz, Lucas J., Xu, Yang, Caldo, Kristian Mark P., Garroway, Colin J., Singer, Stacy D., Chen, Guanqun
Triacylglycerols have important physiological roles in photosynthetic organisms, and are widely used as food, feed and industrial materials in our daily life. Phospholipid:diacylglycerol acyltransferase (PDAT) is the pivotal enzyme catalyzing the acyl‐CoA‐independent biosynthesis of...
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2020-06-13
Subedi, Udaya, Ozga, Jocelyn A., Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
The demand for vegetable oils is increasing at a rapid pace due to our ever-expanding population, growing global affluence, changing dietary choices, and the need for renewable plant-derived resources. However, oilseed production is negatively impacted by unpredictable environmental conditions...
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Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue
Download2020-02-11
Wijekoon, Champa, Singer, Stacy D., Weselake, Randall J., Petrie, James R., Chen, Guanqun, Singh, Surinder, Eastmond, Peter J., Acharya, Surya N.
Alfalfa (Medicago sativa L.) is the most widely grown perennial forage crop and is a close relative of the model diploid legume Medicago truncatula Gaertn. However, use of alfalfa leads to substantial greenhouse gas emissions and economic losses related to inefficiencies in rumen fermentation....
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Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity
Download2018-12-17
Xu, Yang, Caldo, Kristian Mark P., Holic, Roman, Mietkiewska, Elzbieta, Ozga, Jocelyn, Rizvi, Syed Masood, Chen, Guanqun, Weselake, Randall
Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which in turn serves as the major acyl donor for various lipid metabolic pathways. Increasing the size of acyl-CoA pool by enhancing LACS activity appears to be a useful...
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Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesis
Download2019-12-31
Wijekoon, Champa P., Singer, Stacy D., Weselake, Randall J., Petrie, James R., Singh, Surinder, Jayawardhane, Kethmi N., Shah, Saleh, Chen, Guanqun, Eastmond, Peter J., Acharya, Surya N.
Alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite the high protein content, drawbacks to their use exists, including inefficient protein digestibility and energy use efficiency in the ruminant...
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Physaria fendleri and Ricinus communis LCAT-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
Download2020-10-27
Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Mietkiewska, Elzbieta, Greer, Michael S., Tian, Bo, Dyer, John M., Smith, Mark, Zhou, Xue-Rong, Qiu, Xiao, Weselake, Randall J., Chen, Guanqun
Producing hydroxy fatty acids (HFAs) in transgenic crops represents a promising strategy to meet our demands for specialized plant oils with industrial applications. The expression of Ricinus communis (castor) OLEATE 12-HYDROXYLASE (RcFAH12) in Arabidopsis has resulted in only...
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Seed-specific down-regulation of Arabidopsis CELLULOSE SYNTHASE 1 or 9 reduces seed cellulose content and differentially affects carbon partitioning
Download2020-04-20
Jayawardhane, Kethmi N., Singer, Stacy D., Ozga, Jocelyn A., Rizvi, Syed Masood, Weselake, Randall J., Chen, Guanqun
Key message Seed-specific down-regulation of AtCESA1 and AtCESA9, which encode cellulose synthase subunits, differentially affects seed storage compound accumulation in Arabidopsis. High amounts of cellulose can negatively affect crop seed quality, and, therefore, diverting carbon partitioning...
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The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops
Download2020-08-27
Subedi, Udaya, Jayawardhane, Kethmi N., Pan, Xue, Ozga, Jocelyn, Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
A continuous rise in demand for vegetable oils, which comprise mainly the storage lipid triacylglycerol, is fueling a surge in research efforts to increase seed oil content and improve fatty acid composition in oilseed crops. Progress in this area has been achieved using both conventional...