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Permanent link (DOI): https://doi.org/10.7939/R32N4ZX5N

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Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice Open Access

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Author or creator
Tang, Xiaoyun
Benesch, Matthew G. K.
Dewald, Jay
Zhao, Yuan Y.
Patwardhan, Neeraj
Santos, Webster L.
Curtis, Jonathan M.
McMullen, Todd P. W.
Brindley, David N.
Additional contributors
Subject/Keyword
Thyroid Cancer
Epidermal Growth Factor Receptor
Autotaxin
G-Protein-Coupled Receptors
Cell Migration
Lysophosphatidate
Breast Cancer
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Lipid phosphate phosphatase-1 (LPP1) degrades lysophosphatidate (LPA) and attenuates receptor-mediated signaling. LPP1 expression is low in many cancer cells and tumors compared with normal tissues. It was hypothesized from studies with cultured cells that increasing LPP1 activity would decrease tumor growth and metastasis. This hypothesis has never been tested in vivo. To do this, we inducibly expressed LPP1 or a catalytically inactive mutant in cancer cells. Expressing active LPP1 increased extracellular LPA degradation by 5-fold. It also decreased the stimulation of Ca2+ transients by LPA, a nondephosphorylatable LPA1/2 receptor agonist and a protease-activated receptor-1 peptide. The latter results demonstrate that LPP1 has effects downstream of receptor activation. Decreased Ca2+ mobilization and Rho activation contributed to the effects of LPP1 in attenuating the LPA-induced migration of MDA-MB-231 breast cancer cells and their growth in 3D culture. Increasing LPP1 expression in breast and thyroid cancer cells decreased tumor growth and the metastasis by up to 80% compared with expression of inactive LPP1 or green fluorescent protein in syngeneic and xenograft mouse models. The present work demonstrates for the first time that increasing the LPP1 activity in three lines of aggressive cancer cells decreases their abilities to produce tumors and metastases in mice.
Date created
2014
DOI
doi:10.7939/R32N4ZX5N
License information
© 2014 by the American Society for Biochemistry and Molecular Biology, Inc.
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Citation for previous publication
Tang, X., Benesch, M. G. K., Dewald, J., Zhao, Y. -Y., Patwardhan, N., Santos, W. L., Curtis, J. M., McMullen, T. P. W., & Brindley, D. N. (2014). Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice. Journal of Lipid Research, 55(11), 2389-2400.  http://dx.doi.org/10.1194/jlr.M053462

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