Activation of the Na+/H+ exchanger promoter by the transcription factor AP-2

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  • We have isolated and characterized regions important for expression of the mouse NaGraphic/HGraphic exchanger gene. A 1.1-kilobase fragment upstream of the 5′-untranslated region contains specific DNA motifs characteristic of promoter and enhancer elements including a TATA box, two CAAT boxes, an SP-1 site, a cyclic AMP response element-binding site, and an AP-2-like site. This 1.1-kilobase fragment directs transcription of a luciferase reporter gene in mouse fibroblasts (NIH 3T3) and human Hep G2 cells. Deletion or mutation of an AP-2-like site 100 base pairs from the start site of transcription resulted in loss of most of the reporter plasmid activity. In addition, cotransfection of an AP-2 expression plasmid and the mouse promoter/luciferase plasmid increased the amount of NaGraphic/HGraphic exchanger-directed transcription in AP-2-deficient Hep G2 cells. Moreover, mobility shift analysis indicated that a putative AP-2-binding site is capable of binding purified AP-2 protein and a specific protein from nuclear extracts of NIH 3T3 cells. The results show that the transcription factor AP-2 may play an important role in regulation of transcription of the mouse NaGraphic/HGraphic exchanger gene.

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    Article (Published)
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    © 1995 Jason Dyck et al. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
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    • Dyck, Jason, Silva, Norma Lucena C. L., & Fliegel, Larry. (1995). Activation of the Na+/H+ exchanger promoter by the transcription factor AP-2. Journal of Biological Chemistry, 270(3), 1375-1381.
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