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2018-01-16

Hunter, Carmanah D, Khanna, Neha, Richards, Michele R, Darestani, Reza Rezaei, Zou, Chunxia, Klassen, John S, Cairo, Christopher W.

Recognition of terminal sialic acids is central to many cellular processes, and structural modification of sialic acid can disrupt these interactions. A prominent, naturally occuring, modification of sialic acid is 9-O-acetylation (9-O-Ac). Study of this modification through generation and analysis

-acetylation, and C-5 group (Neu5Gc). Relative rates of substrate cleavage by hNEU were determined using fluorescence spectroscopy and electrospray ionization mass spectrometry. We report that 9-O-acetylation had a significant, and differential, impact on sialic acid hydrolysis by hNEU with general substrate

aglycone. The impact of these minor structural changes to sialic acid on hNEU activity was unexpected, and these results provide evidence of the substantial influence of 9-O-Ac modifications on hNEU enzyme substrate specificity. Furthermore, these findings may implicate hNEU in processes governed by 9-O

2009

Hook, Sarah E., AlZahal, Ousama, McBride, Brian W., Croom, Jim, Steele, Michael A.

structural and functional adaptations of the rumen epithelium. On the final day of the grain challenge, the daily mean ruminal pH was 5.41 ± 0.09 with a minimum of 4.89 and a maximum of 6.31. Ruminal pH was under 5.0 for 130 minutes (2.17 hours) which is characterized as the acute form of ruminal acidosis in

. Rumen papillae from the high grain diet displayed extensive sloughing of the stratum corneum and compromised cell adhesion as large gaps were apparent between cells throughout the strata. This case report represents a rare documentation of how the rumen epithelium alters its function and structure

2007

Savolainen, P., Lundeberg, J., Andersson, L., Rubin C-J, Lindberg J, Fitzsimmons, C., Jensen, P., Kindmark, A.

transcripts, those encoding structural ribosomal proteins were highly enriched and all 15 had lower expression in WL. Conclusion We report the identification of 779 differentially expressed transcripts, several residing within QTL-regions for bone traits. Among differentially expressed transcripts, those

encoding structural ribosomal proteins were highly enriched and all had lower expression levels in WL. In addition, transcripts encoding four translation initiation and translation elongation factor proteins also had lower expression levels in WL, possibly indicating perturbation of protein biosynthesis

2009

Bookey-Bassett, S., Tourangeau, A. E., Pachis, J., Widger, K., Cranley, L.

literature reporting on the structural characteristics of work environments and care provider responses to work environments in institutional long-term (chronic) care settings. Purpose: The purpose of this article was to report how a convenience sample of multidisciplinary care providers working in

2005

Owttrim, George W., Chamot, Danuta, Kujat-Choy, Sonya L., Colvin, Kimberley R.

Rearrangement of RNA secondary structure is crucial for numerous biological processes. RNA helicases participate in these rearrangements through the unwinding of duplex RNA. We report here that the redox-regulated cyanobacterial RNA helicase, CrhR, is a bona fide RNA helicase possessing both RNA

characteristic of RNA helicases. Biochemically, CrhR resembles RecA and related proteins that catalyze strand exchange and branch migration on DNA substrates, a characteristic that is reflected in the recently reported structural similarities between these proteins. The data indicate the potential for CrhR to

2009

Abbott, D. W., Boraston, Alisdair B. , Vocadlo, David J., Macauley, Matthew S.

we describe mechanistic and structural studies of the catalytic domain (SpGH85) of Endo-D that provide compelling support for GH85 enzymes using a catalytic mechanism involving substrate-assisted catalysis. Furthermore, the structure of SpGH85 in complex with the mechanism-based competitive inhibitor

kinetic studies, suggests these enzymes may use an unusual proton shuttle to coordinate effective general acid and base catalysis to aid cleavage of the glycosidic bond. These results collectively provide a blueprint that may be used to facilitate protein engineering of these enzymes to improve their

2023-04-26

Pawan Kumar

as g-C3N4 and C3N5 has been shown to mitigate the photocorrosion problem but the poor oxidizing power of photogenerated holes in CN limits the utility of this approach for photooxidation reactions. We report the synthesis of exfoliated 2D nanosheets of a modified carbon nitride constituted of tris-s

/CN:PDI) was determined to be an excellent photocatalyst for oxidation reactions including photoelectrochemical water splitting, dye decolorization and the photocatalytic conversion of benzyl alcohol to benzaldehyde. Extensive structural characterization using HR-TEM, Raman, XPS, etc., confirmed wrapping

2019-02-01

Schramm, David G., Galovan, Adam M., Futris, Ted G., Kanter, Jeremy B.

training in RME, we used structural equation modeling with participant self-efficacy, utility, and confidence as predictors of application of RME concepts at 2 months post-training. We also assessed how application of RME concepts at 2 months predicted self-efficacy, confidence, and application at 6 months

. Results: Only the combined effect of both higher self-efficacy and higher utility was related to applying concepts at 2 months. Those who apply the concepts at 2 months are more likely both to report higher confidence at 6 months and to apply the concepts at 6 months. Conclusions: Evaluations of trainings