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Spring 2011

Lo, Andy

This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...

Fall 2012

Xu, Mingguo

The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...

Spring 2010

Lu, Qingye

Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...

Spring 2023

Siva Piragasam, Ramanaguru

Over the last few decades, the field of proteomics has developed rapidly owing to advancements in genomic and transcriptomic technologies contributing to the compilation of vast quantities of protein sequence libraries. Proteomics, the study of global protein composition in a biological system,...

Spring 2023

Khodabocus, Ibrahim

Plants require periods of light and darkness to grow and develop properly. In plants, the timing of daily events is facilitated by the circadian clock. Most eukaryotes possess a circadian circuit that is entrained by different inputs such as light and temperature. Here, I show that the only...

Fall 2020

Chan, Calvin J

The Golgi complex resides at the center of the cellular trafficking pathway, where it functions in the modification, sorting, and trafficking of over one third of human proteins to their correct cellular destination. Initiation of vesicle formation at the Golgi requires activation of small...

Fall 2021

Araya, Luam Ellen

Caspases are a family of enzymes that regulate biological processes, such as inflammation and programmed cell death, through proteolysis. For example, in the intrinsic pathway of apoptosis, cell death signaling involves cytochrome c release from the mitochondria, which leads to the activation of...

Fall 2024

Saini, Arashdeep S

In response to hypoxic conditions, tumours secrete pro-angiogenic factors that stimulate the development of new blood vessels. Given the critical role of angiogenesis in tumour progression, therapies counteracting cancer neovascularization have been developed. However, clinical studies have shown...

Fall 2023

Moussa, Eman W

Viral infection and the resultant cellular stress can trigger significant changes to the cellular host proteome. Viral proteases essential to viral replication have been shown to cleave human host protein substrates as a mechanism of immune response evasion. The severe acute respiratory syndrome...

2017-01-30

Albert Remus Rosana

Several strains of Bacillus spp. were isolated from across Algeria, the largest African country. The strains were bioprospected from several economically-relevant environments such as oil-mining sites, salty lakes, coal mines, deserts and oil-contaminated water basins. Hundreds of isolates were...