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Omics-based bioactive and drug discovery in Bacillus spp.

  • Author(s) / Creator(s)
  • Several strains of Bacillus spp. were isolated from across Algeria, the largest African country. The strains were bioprospected from several economically-relevant environments such as oil-mining sites, salty lakes, coal mines, deserts and oil-contaminated water basins. Hundreds of isolates were extensively screened for extracellular enzyme and lipid-emulsifying properties and pathogen-inhibition activities. Potent strains were further subjected to both genomics and proteomics-metabolomics based-pipelines for gene-cluster and bioactive peptide discovery, respectively. Whole genome shotgun sequencing revealed new strains of Bacillus spp. namely: the insecticidal B. thuringensis; the biosurfactant-producing B. amyloliquefaciens; the bacteriocin-producing B. paralicheniformis; the food borne-pathogen B. cereus and the soil-borne pathogen B. anthacis. Cluster gene analyses revealed an extensive repertoire of secondary metabolite families encoded in the genome of these new Bacillus strains such as bacteriocins, terpenes, lassopeptides, lipopeptides, homoserine lactones, and siderophores. These bioinformatically predicted gene inventories were coupled with metabolomics platforms to confirm a number of biotechnologically relevant molecules. Nuclear magnetic resonance spectroscopy (NMR) and liquid chromatography-mass spectrometry (LC-MS) identified several potent bioactives such as fengycins, lantipeptides and microcins with strong bactericidal activity against common food borne-pathogens such as E. coli, Salmonella and S. aureus. Here, we will present the direct biotechnological application of genomics and targeted metabolomics in microbial bioactive predictions and drug discovery.

  • Date created
    2017-01-30
  • Subjects / Keywords
  • Type of Item
    Conference Poster
  • DOI
    https://doi.org/10.7939/R3J960N4N
  • License
    Public Domain Mark 1.0
  • Language
  • Additional contributors
  • Citation for previous publication
    • Project presented (poster and 3MT) at the RE Peter Biology Conference 2017, Dept of Biological Sciences, University of Alberta, Jan 30-31, 2017 and 24th Metagenomics and Genomics Workshop, Joint Genome Institute-DOE, Walnut Creek, California, Feb 27-Mar3, 2017