Search
Skip to Search Results- 33Mass-spectrometry
- 22Metabolomics
- 8Plasma spectroscopy
- 6Chemical isotope labeling
- 5LC-MS
- 4Isotope labeling
- 1Alexander, Anthony John.
- 1Chan, Wing Tat.
- 1Chen, Lu
- 1Curle, Jared P
- 1Dawe, Margot Renee
- 1De Souza, Andrea
-
Spring 2015
The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass...
-
Fall 2018
Metabolomics is the ultimate reflection of organisms influenced by both genetic and environmental factors. Compared with other omics areas, it is the most appropriate and closest description of phenotype, which is the comprehensive characteristics of an organism. It is a powerful tool for global...
-
Development and Application of Chemical Isotope Labeling Methods and Metabolite Identification Solution for Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2018
Metabolomics, the comprehensive analysis of small molecules in biological specimens, has become an emerging and indispensable tool for systems biology and clinical research. Liquid chromatography-mass spectrometry (LC-MS) is a dominant analytical platform for metabolomics, featuring high...
-
Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2016
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
-
Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
DownloadFall 2016
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
-
Development and application of mass spectrometry for bioanalysis of proteins and metabolites
DownloadSpring 2011
The proteome, a hybrid term from protein and genome, is the total set of proteins expressed by a genome in a cell, tissue, or organism at a given time. Proteomics, the study of the proteome, is concerned with a variety of topics such as identity, quantity, activity, structure, protein-protein...
-
Development and Applications of Chemical Isotope Labeling Methods for Metabolome Sample Normalization and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2015
Metabolomics refers to the global study of the metabolome of a biological system where comprehensive and systematic profiling of all metabolites in a given biological sample (i.e., the metabolome) is performed. As the end product of biological processes, the status of metabolites could reflect...
-
Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence Characterization
DownloadFall 2013
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
-
Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...