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Skip to Search Results- 9Owttrim, George W.
- 5Chamot, Danuta
- 4Owttrim, George W.
- 2Coleman, John R.
- 2Colvin, Kimberley R.
- 2Rosana, Albert R. R.
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A LexA-related protein regulates redox-sensitive expression of the cyanobacterial RNA helicase, crhR
Download2006
Colvin, Kimberley R., Owttrim, George W. , Patterson-Fortin, Laura M.
Expression of the cyanobacterial DEAD-box RNA helicase, crhR , is regulated in response to conditions, which elicit reduction of the photosynthetic electron transport chain. A combination of electrophoretic mobility shift assay (EMSA), DNA affinity chromatography and mass spectrometry identified...
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1999
Owttrim, George W., Magee, Wendy C., Yu, Esther, Chamot, Danuta
The ability to modify RNA secondary structure is crucial for numerous cellular processes. We have characterized two RNA helicase genes, crhB and crhC, which are differentially expressed in the cyanobacterium Anabaena sp. strain PCC 7120. crhC transcription is limited specifically to cold shock...
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Adsorption of Biologically Critical Transition Elements to the Marine Cyanobacterium Synechococcus SP PCC 7002
Download2016-09-26
Bishop, Brendan A., Flynn, Shannon L., Robbins, Leslie J., Warchola, Tyler J., Owttrim, George W., Alessi, Daniel S., Konhauser, Kurt O.
It has been demonstrated that microbial biomass plays an important role in marine trace element cycling. The influence of microbes may be attributed to the dominantly negative surface charge of bacteria at pH values characteristic of seawater. Recent work has begun to more fully explore how...
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Autoregulation of RNA helicase expression in response to temperature stress in Synechocystis spCC 6803
Download2012
Owttrim, George W., Chamot, Danuta, Rosana, Albert R. R.
RNA helicases are ubiquitous enzymes whose modification of RNA secondary structure is known to regulate RNA function. The pathways controlling RNA helicase expression, however, have not been well characterized. Expression of the cyanobacterial RNA helicase, crhR, is regulated in response to...
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Conditional, temperature-induced proteolytic regulation of cyanobacterial RNA helicase expression
Download2014
Owttrim, George W., Tarassova, Oxana S., Chamot, Danuta
Conditional proteolysis is a crucial process regulating the abundance of key regulatory proteins associated with the cell cycle, differentiation pathways, or cellular response to abiotic stress in eukaryotic and prokaryotic organisms. We provide evidence that conditional proteolysis is involved...
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Cyanobacterial RNA Helicase CrhR Localizes to the Thylakoid Membrane Region and Cosediments with Degradosome and Polysome Complexes in Synechocystis sptrain PCC 6803
Download2016
Fahlman, Richard P., Owttrim, George W. , Rosana, Albert R. R., Whitford, Denise S.
The cyanobacterium Synechocystis sp. strain PCC 6803 encodes a single DEAD box RNA helicase, CrhR, whose expression is tightly autoregulated in response to cold stress. Subcellular localization and proteomic analysis results indicate that CrhR localizes to both the cytoplasmic and thylakoid...
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Cyanobacterial biofactories: combining evolved and synthetic genetic regulatory mechanisms to yield carbon-neutral bioproducts
Download2017-01-01
Brand, Logan A., Owttrim, George W.
While cyanobacteria hold the promise of producing useful bioproducts from little more than sunlight and carbon dioxide, their economic feasibility hinges on developing better mechanisms to manipulate gene expression in order to divert photosynthate towards product formation. A recent report...
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1987
Owttrim, George W., Coleman, John R.
A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as...
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1986
Owttrim, George W., Colman, Brian.
Phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31) was purified 100-fold from the cyanobacterium Coccochloris peniocystis with a yield of 10%. A single isozyme was found at all stages of purification, and activity of other beta-carboxylase enzymes was not detected. The apparent molecular weight...
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2006
RNA helicases function as molecular motors that rearrange RNA secondary structure, potentially performing roles in any cellular process involving RNA metabolism. Although RNA helicase association with a range of cellular functions is well documented, their importance in response to abiotic stress...