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- 3ESI-MS
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Application of Electrospray Ionization Mass Spectrometry to Study Protein-Ligand Interactions and Enzyme Kinetics
DownloadFall 2015
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) to study the noncovalent protein-ligand interactions and also investigate the kinetic of enzymatic reactions. The rate of glycolipids cleavage by the human neuraminidase 3 (hNEU3) was...
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Fall 2013
This thesis describes the studying of electrospray ionization (ESI) process and the application of the direct ESI-MS assay to study noncovalent carbohydrate-protein interactions in vitro. Protein unfolding induced by ESI process was first investigated. It is proposed that the Coulombic repulsion...
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Characterization of glycosyltransferases and glycosidases using electrospray mass spectrometry
DownloadFall 2011
Carbohydrates are one of the major classes of organic compounds found in nature and living organisms, and the diverse roles of carbohydrates are crucial for most life forms. The transformation and degradation of glycans are effectively regulated by carbohydrate-modifying enzymes. This thesis...
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Characterization of Noncovalent Interactions between Human Milk Oligosaccharides and Proteins
DownloadFall 2018
This thesis focuses on the characterization of noncovalent interactions between human milk oligosaccharides (HMOs) and proteins using electrospray ionization mass spectrometry (ESI-MS) based assays. HMOs play critical roles in protecting infants from viral and bacterial infections through...
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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Investigating Protein-Carbohydrate Interactions with Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS)
DownloadFall 2014
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to investigating protein-carbohydrate interactions is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin (CTB5) and Shiga toxin type 1 (Stx1B5) and a fragment of Clostridium...
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Study of Non-Covalent Multisubunit Protein-Carbohydrate Interactions by Electrospray Ionization Mass Spectrometry
DownloadFall 2013
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) based techniques to investigate protein-carbohydrate interactions in vitro. A catch-and-release electrospray ionization mass spectrometry (CaR-ESI-MS) assay was developed for the...
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Study of Non-Covalent Protein-Carbohydrate Interactions by Nanoelectrospray Ionization Mass Spectrometry and Surface Plasmon Resonance
DownloadFall 2015
This thesis describes the application of electrospray ionization mass spectrometry (ESI-MS) techniques to identify and quantify antibody-antigen interactions in vitro. Also potential application of nanodiscs (ND) as membrane model in ESI-MS and surface plasmon resonance (SPR) spectroscopy has...
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Studying Protein–Glycolipid Interactions and Membrane Peptides and Proteins using Electrospray Ionization Mass Spectrometry and Model Membranes
DownloadFall 2018
Membrane systems, including glycolipids (GLs) and membrane peptides and proteins (MPs), play an important role in many cellular processes, such as singling, cellular recognition, transportation, and energy conversion. However, the amphipathic nature of GLs and MPs makes their analysis...