Study of Non-Covalent Protein-Carbohydrate Interactions by Nanoelectrospray Ionization Mass Spectrometry and Surface Plasmon Resonance

• Author / Creator

  Nikjah, Sanaz

• This thesis describes the application of electrospray ionization mass spectrometry (ESI-MS) techniques to identify and quantify antibody-antigen interactions in vitro. Also potential application of nanodiscs (ND) as membrane model in ESI-MS and surface plasmon resonance (SPR) spectroscopy has been investigated.
  A direct nanoelectrospray mass spectrometry assay (nanoESI-MS) was developed for quantification of specific binding between a monoclonal antibody (mAb) and its cognate antigen. A qualitative investigation was performed on cross-reactivity of antibodies. NanoESI-MS was also employed to investigate the effects of fatty acid chain lengths on its degree of incorporation into ND. The results can be utilized for synthesis purposes where biologically important carbohydrates require a proper carbon chain for incorporation into ND.
  ND technology was also employed to investigate protein-ligand interaction using SPR spectroscopy. The results open up further investigation on the effect of the position of ganglioside binding sites incorporated into ND and ability of their binding to the receptors in SPR.

• Subjects / Keywords

  • Antibody
  • Mass-spectrometry
  • Protein-Carbohydrate Interactions

• Graduation date

  Fall 2015

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3513V72B

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.