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Carbohydrate-Protein Interactions Studied Using Electrospray Ionization Mass Spectrometry

  • Author / Creator
    Lin, Hong
  • This thesis describes the studying of electrospray ionization (ESI) process and the application of the direct ESI-MS assay to study noncovalent carbohydrate-protein interactions in vitro. Protein unfolding induced by ESI process was first investigated. It is proposed that the Coulombic repulsion between the negatively charged residues and liquid/droplet surface charge under certain ESI conditions can induce unfolding of acidic proteins. A deleterious non-uniform response factors phenomenon induced by high molecular weight molecules and complexes in the application of direct ESI-MS assay was also investigated. It is possibly due to the reduction in the number of available surface sites in the ESI droplets upon introduction of large solute and increased competition between protein and the more hydrophilic carbohydrate-protein complex for these sites. Direct ESI-MS assay was also utilized to investigate the stepwise binding of the GM1 pentasaccharide (GM1os) to the cholera toxin B subunit homopentamer and to elucidate positive binding cooperativity.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3H677
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • Klassen, John (Chemistry)
  • Examining committee members and their departments
    • McDermott, Mark (Chemistry)
    • Petersen, Nils (Chemistry)