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Skip to Search Results- 5Cryopreservation
- 3Cryoprotectant agents
- 3TF-1
- 2Articular cartilage
- 2Chondrocytes
- 2Computer modeling
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Characterization of cryobiological responses in TF-1 cells using interrupted freezing procedures
Download2014-09-22
McGann, L. E., Elliott, J. A., Ross-Rodriguez, L. U.
Cryopreservation currently is the only method for long-term preservation of cellular viability and function for uses in cellular therapies. Characterizing the cryobiological response of a cell type is essential in the approach to designing and optimizing cryopreservation protocols. For cells used...
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2010
Law, G. K., Elliott, J. A., Forbes, J. F., Weiss, A. D., McGann, L. E., Jomha, N. M.
Large articular cartilage defects have proven difficult to treat and often result in osteoarthritis of the affected joint. Cryopreservation of articular cartilage can provide an increased supply of tissues for osteochondral allograft but cryoprotective agents are required; however, few studies...
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2012
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...
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Investigating cryoinjury using simulations and experiments: 1. TF-1 cells during two-step freezing (rapid cooling interrupted with a hold time)
Download2014-09-22
McGann, L. E., Ross-Rodriguez, L. U., Elliott, J. A.
There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...
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Investigating cryoinjury using simulations and experiments: 2. TF-1 cells during graded freezing (interrupted slow cooling without hold time)
Download2014-09-22
McGann, L.E., Ross-Rodriguez, L.U., Elliott, J.A.
Cryopreservation plays a key role in the long-term storage of native and engineered cells and tissues for research and clinical applications. The survival of cells and tissues after freezing and thawing depends on the ability of the cells to withstand a variety of stresses imposed by the...
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2014-09-26
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability...