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- 5Weselake, Randall J.
- 5Xu, Yang
- 4Mietkiewska, Elzbieta
- 3Caldo, Kristian Mark P.
- 3Ozga, Jocelyn
- 22Graduate and Postdoctoral Studies (GPS), Faculty of
- 22Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 9Agricultural, Food and Nutritional Science, Department of
- 9Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 2Deyholos, Michael (Biological Sciences)
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Davis, Corey (Biological Sciences)
- 1Dosdall, Lloyd M. (Agricultural, Food and Nutritional Science)
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Introgression of Clubroot Resistance from Brassica oleracea into B. napus Canola through Interspecific Cross
DownloadSpring 2022
Clubroot disease caused by Plasmodiophora brassicae Woronin is a serious threat to canola production in Canada. Most of the available clubroot-resistant canola cultivars carry major resistance genes of the Brassica A genome. However, the ineffectiveness of this type of resistance has been...
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Molecular and functional characterization of sn-glycerol-3-phosphate acyltransferase of plants
DownloadSpring 2011
sn-Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the acylation of sn-1 position of sn-glycerol-3-phosphate to produce lysophosphatidic acid and Coenzyme A. GPATs are involved in several lipid synthetic pathways and play important physiological roles in plant development. The present...
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Spring 2022
Clubroot, caused by the soilborne pathogen Plasmodiophora brassicae, is a threat to cruciferous crops worldwide and an important disease of canola (Brassica napus L.) in Canada. At present, pathotypes of P. brassicae are distinguished phenotypically based on their virulence patterns on host...
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...