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Skip to Search Results- 13Cryopreservation
- 9Articular cartilage
- 7Vitrification
- 6Porcine
- 4Cryoprotectant agents
- 3Chondrocytes
- 12Graduate and Postdoctoral Studies (GPS), Faculty of
- 12Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 11Chemical and Materials Engineering, Department of
- 11Chemical and Materials Engineering, Department of/Research Articles and Materials (Chemical and Materials Engineering)
- 7Surgery, Department of
- 7Surgery, Department of/Orthopaedic Surgery
- 1Adesida, Adetola B (Surgery)
- 1Adesida, Adetola B. (Surgery)
- 1Bagnall, Keith (Department of Surgery)
- 1Dr. Michael Dyck (Department of Agricultural, Food, and Nutritional Science)
- 1Elliott, Janet A W (Chemicals and Material Engineering)
- 1Elliott, Janet A. W. (Chemical & Materials Engineering and Laboratory Medicine & Pathology)
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Investigating cryoinjury using simulations and experiments: 1. TF-1 cells during two-step freezing (rapid cooling interrupted with a hold time)
Download2014-09-22
McGann, L. E., Ross-Rodriguez, L. U., Elliott, J. A.
There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...
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1988
Plastow, G. S., Mileham, A. J., Siggens, K. W.
A 3.8 kb male specific fragment was observed following agarose gel etectrophoresis of porcine DNA digested with Sph I. DNA was recovered from this region of a gel using DE-81 paper and ligated to Sph I digested pUC18. Plasmids carrying male specific sequences were identified by their differential...
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Fall 2009
Islet transplantation has the potential to be an effective treatment for patients with type 1 diabetes. However, a shortage of human donor islets and the need for continuous immunosuppressive therapy currently limit this therapy to patients with brittle type 1 diabetes. Neonatal pigs may...
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Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation
DownloadSpring 2011
Loading vitrifiable concentrations of cryoprotective agents is an important step for cryopreservation of biological tissues by vitrification for research and transplantation purposes. This may be done by immersing the tissue in a cryoprotective agent (CPA) solution, and increasing the...
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Fall 2015
Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no cure for osteoarthritis, and the current treatments are all lacking in some form or another. One treatment which attempts to prevent degeneration of...
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2014-09-26
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability...
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Fall 2015
Cryopreserving cells for human health therapeutics is a multidisciplinary process with great complexity. Cryopreservation of human umbilical vein endothelial cells (HUVECs) has facilitated vascular biology research since they were first isolated in 1973 [59]; however identifying key variables to...
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Fall 2016
Articular cartilage injury is a major risk factor for the development of osteoarthritis, a condition that results in significant patient morbidity and substantial cost to the healthcare system. Due to the limited capacity of articular cartilage to regenerate, early intervention is required to...
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Statistical prediction of the vitrifiability and glass stability of multi-component cryoprotective agent solutions.
Download2010
Forbes, J. F., Law, G. K., Weiss, A. D., Scheuerman, A., McGann, L. E., Elliott, J. A., Jomha, N. M.
Long-term biologic storage of articular cartilage has proven elusive due to cellular degradation over time or acute damage during attempts at cryopreservation. Vitrification is one option that may result in successful cryopreservation but difficulty with cryoprotective agent (CPA) toxicity at...
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Spring 2013
Early embryonic development is a dynamic period that requires precise and timely interaction between the developing embryo and the maternal environment. A series of studies were carried out in order to identify the vital components of this environment and to understand their temporal and spatial...