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Skip to Search Results- 9Chen, Guanqun
- 8Xu, Yang
- 5Weselake, Randall J.
- 4Caldo, Kristian Mark P.
- 4Mietkiewska, Elzbieta
- 3Greer, Michael S.
- 13Agricultural, Food and Nutritional Science, Department of
- 13Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 13Graduate and Postdoctoral Studies (GPS), Faculty of
- 13Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
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Effects of nutritional management on production performance and rumen fermentation in dairy cows during calving transition
DownloadSpring 2019
Dairy cows usually experience negative energy balance during the calving transition period (3 weeks before to 3 weeks after parturition) due to the rapid increase in nutrient demands for milk production. In order to compensate for the energy deficits, diets rich in highly fermentable...
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Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity
Download2018-12-17
Xu, Yang, Caldo, Kristian Mark P., Holic, Roman, Mietkiewska, Elzbieta, Ozga, Jocelyn, Rizvi, Syed Masood, Chen, Guanqun, Weselake, Randall
Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which in turn serves as the major acyl donor for various lipid metabolic pathways. Increasing the size of acyl-CoA pool by enhancing LACS activity appears to be a useful...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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Improving the production of punicic acid in baker’s yeast by engineering genes in acyl channeling processes and adjusting precursor supply
Download2021-01-01
Wang, Juli, Xu, Yang, Holic, Roman, Yu, Xiaochen, Singer, Stacy D., Chen, Guanqun
ABSTRACT Punicic acid (PuA) is a high-value edible conjugated fatty acid with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, feeding and oleochemical industries. Since the production of PuA is severely limited by the fact that its natural source...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Mammalian and Saccharomyces cerevisiae ER-mitochondria contact site regulation by small Rab GTPases and ER folding assistants
DownloadFall 2021
Membrane-bound organelles allow eukaryotes to compartmentalize components and processes in a highly organized manner. Organelles can communicate with one another through membrane contact sites (MCS): membrane appositions 10-50nm apart. MCS were not widely accepted as bona fide sites of organelle...
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Mechanisms of inactivation of Candida humilis and Saccharomyces cerevisiae by pulsed electric fields
Download2017-06-01
Ou, Qi-Xing, Nikolic-Jaric, Marija, Gänzle, Michael
Aims This study aimed to determine how electric field strength, pulse width and shape, and specific energy input relate to the effect of pulsed electric fields (PEF) on viability and membrane permeabilization in Candida humilis and Saccharomyces cerevisiae suspended in potassium phosphate...