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Skip to Search Results- 25Brassica napus
- 17Saccharomyces-cerevisiae
- 12DGAT
- 7Canola
- 7Triacylglycerol biosynthesis
- 6Enzyme kinetics
- 12Chen, Guanqun
- 11Xu, Yang
- 9Weselake, Randall J.
- 6Caldo, Kristian Mark P.
- 5Pan, Xue
- 4Mietkiewska, Elzbieta
- 34Graduate and Postdoctoral Studies (GPS), Faculty of
- 34Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 17Agricultural, Food and Nutritional Science, Department of
- 17Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 6Biological Sciences, Department of
- 6Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 21Department of Agricultural, Food, and Nutritional Science
- 4Department of Biological Sciences
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Botany
- 1Department of Chemical Engineering
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Allen Good (Biological Sciences)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Dosdall, Lloyd M. (Agricultural, Food and Nutritional Science)
- 1Dsupervisor: Dr. Habibur Rahman, AFNS Department, co-supervisor: Dr. Rong-Cai Yang (AFNS Department)
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Download2007
Pozniakovsky, A., Hyman, A.A., Stirling, P.C., Takhar, K.S., Srayko, M., Lerou, M.R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators of the cell cycle. Here, we demonstrate that the essential and conserved eukaryotic phosducin-like...
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Genetic and molecular analysis of clubroot resistance in canola introgressed from rutabaga cvs. Polycross and Brookfield
DownloadFall 2022
Clubroot disease caused by Plasmodiophora brassicae is a serious threat to canola (Brassica napus) production. The evolution of new pathotypes has rendered available resistances ineffective and has necessitated the introgression of new resistance into canola and furthering our understanding of...
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Inactivation of Saccharomyces cerevisiae and Candida humilis in Potassium Phosphate Buffer by Pulsed Electric Fields
DownloadSpring 2016
Pulsed electric field (PEF) is a promising athermal food preservation technology, having the potential to provide a better balance between food safety and food quality. But the high cost of PEF treatment has limited the commercial application of this technology. The aim of this MSc thesis...