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- 17Saccharomyces-cerevisiae
- 12DGAT
- 10Triacylglycerol
- 7Canola
- 7Triacylglycerol biosynthesis
- 14Chen, Guanqun
- 12Xu, Yang
- 11Weselake, Randall J.
- 7Caldo, Kristian Mark P.
- 6Mietkiewska, Elzbieta
- 6Pan, Xue
- 35Graduate and Postdoctoral Studies (GPS), Faculty of
- 35Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 21Agricultural, Food and Nutritional Science, Department of
- 21Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 7Biological Sciences, Department of
- 7Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 21Department of Agricultural, Food, and Nutritional Science
- 4Department of Biological Sciences
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Botany
- 1Department of Chemical Engineering
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Allen Good (Biological Sciences)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Dosdall, Lloyd M. (Agricultural, Food and Nutritional Science)
- 1Douglas, Donna (Department of Surgery)
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Evaluation of Spring Canola (Brassica Napus) Lines Derived from Rutabaga (Brassica Napus Var. Napobrassica) X Canola Crosses for Agronomic and Seed Quality Traits and Heterosis
DownloadSpring 2018
Brassica napus L. (AACC, 2n = 38) canola is the most extensively cultivated Brassica oilseed crop in the world; it is one of the most important field crops in Canada. The narrow genetic diversity in this crop is considered one of the major hindrances for continued improvement of this crop for...
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Download2007
Pozniakovsky, A., Hyman, A.A., Stirling, P.C., Takhar, K.S., Srayko, M., Lerou, M.R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators of the cell cycle. Here, we demonstrate that the essential and conserved eukaryotic phosducin-like...
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Genetic and molecular analysis of clubroot resistance in canola introgressed from rutabaga cvs. Polycross and Brookfield
DownloadFall 2022
Clubroot disease caused by Plasmodiophora brassicae is a serious threat to canola (Brassica napus) production. The evolution of new pathotypes has rendered available resistances ineffective and has necessitated the introgression of new resistance into canola and furthering our understanding of...
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...