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- 12Xu, Yang
- 9Weselake, Randall J.
- 7Caldo, Kristian Mark P.
- 5Mietkiewska, Elzbieta
- 5Pan, Xue
- 16Agricultural, Food and Nutritional Science, Department of
- 16Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 12Graduate and Postdoctoral Studies (GPS), Faculty of
- 12Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 5Oil Sands Research and Information Network (OSRIN)
- 5Oil Sands Research and Information Network (OSRIN)/AOSERP Reports
- 2Vinebrooke, Rolf (Biological Sciences)
- 1Ania C. Ulrich, Civil and Environmental Engineering Department
- 1Case, Rebecca (Biological Sciences)
- 1Case, Rebecca J (Biological Sciences)
- 1Chen, Jie (Electrical and Computer Engineering, Biomedical Engineering)
- 1Douglas, Donna (Department of Surgery)
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2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
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Characterization of Type-2 Diacylglycerol Acyltransferases in the Green Microalga Chromochloris zofingiensis
Download2018-12-13
Xu, Yang, Falarz, Lucas, Chen, Guanqun
Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of the acyl-CoA-dependent TAG biosynthesis and thus is a key target for manipulating oil production in microalgae. The microalga Chromochloris zofingiensis can accumulate substantial amounts of triacylglycerol (TAG) and...
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2022-06-01
Samadi, Zahra, Mehdizadeh-Allaf, Malihe, Saifi, Reza, De Groot, Christopher T., Peerhossaini, Hassan
Mixing displays a major role in the growth of photosynthesis microorganisms in active suspensions: it gives access to nutrients, improves the microorganisms’ exposure to light, and homogenizes temperature distribution in photobioreactors (PBRs). Therefore, mixing is an essential factor in the...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Fall 2023
Global melting of mountain glaciers is altering downstream ecosystems. As glaciers disappear, downstream water temperatures are rising while turbidity and nutrient concentrations decline. Here, knowledge gaps exist concerning how these abiotic changes will affect primary producers in glacial...
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....