Search
Skip to Search Results- 13Etalons
- 10Stimuli-Responsive Polymers
- 5Photonic Materials
- 4Phase-Transition
- 3Protein-Concentration
- 2Biosensing
-
Poly (N-isopropylacrylamide) microgel-based etalons for the label-free quantitation of estradiol-17β in aqueous solutions and milk samples
Download2018-01-01
Jiang, Yaxin, Colazo, Marcos G., Serpe, Michael J.
A novel estradiol-17β (E2) biosensor was constructed from poly(N-isopropylacrylamide) (pNIPAm) microgel-based etalons by modification of their outermost Au layer with an E2 binding 75-mer DNA aptamer. When E2 is not present in the solution, the aptamer forms a loose/linear structure that allows...
-
Isolation of RNA from a mixture and its detection by utilizing a microgel-based optical device
Download2018-01-01
Islam, Molla R., Azimi, Shakiba, Teimoory, Faranak, Loppnow, Glen R., Serpe, Michael J.
In this investigation, we show that RNA can be separated from a solution containing DNA and RNA and the isolated RNA can be detected using poly (N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) microgel-based optical devices (etalons). The isolation of RNA was accomplished...
-
Enzyme-assisted polymer film degradation-enabled biomolecule sensing with poly (N-isopropylacrylamide)-based optical devices
Download2018-01-01
Zhang, Wei, Wei, Menglian, Carvalho, Wildemar S. P., Serpe, Michael J.
A biosensor for mouse Immunoglobulin G (IgG) was generated from responsive polymer-based interference filters (etalons). To accomplish this, an excess amount of alkaline phosphatase-modified goat anti-mouse IgG (AP-GAM, F(ab’)2 fragment specific to mouse IgG) was added to mouse IgG, and allowed...
-
2017-01-01
Zhang, Qiang, Colazo, Juan, Berg, Darren, Mugo, Samuel M., Serpe, Michael J.
Nanogels with a biomolecular coating (biocoating) were shown to be capable of triggered delivery of anticancer drug Doxorubicin. The biocoating was formed utilizing binding between glycogen and the tetra-functional lectin Concanavalin A, which can be triggered to disassemble (and release) upon...
-
2016
Li, Xue, Serpe, Michael J., Gao, Yongfeng
Poly (N-isopropylacrylamide) (pNIPAm)-based hydrogels and hydrogel particles (microgels) have been extensively studied since their discovery a number of decades ago. While their utility seems to have no limit, this feature article is focused on their development and application for sensing small...
-
Sequential and controlled release of small molecules from poly(N-isopropylacrylamide) microgel-based reservoir devices
Download2016
Serpe, Michael J., Wong, Ka Y., Ahiabu, Andrews, Gao, Yongfeng
Systems composed of a poly(N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgels (AAc-MG) and poly(N-isopropylacrylamide-3-(acrylamido)phenylboronic acid) (pNIPAm-co-APBA) microgels (APBA-MG) were used to sequentially release small molecules to a system in a controlled and triggered...
-
2016-01-01
Jiang, Yaxin, Colazo, Marcos G., Serpe, Michael J.
Progesterone (P4) is a vital steroid hormone involved in the reproduction cycle and necessary for maintenance of pregnancy in humans and animals. Monitoring P4 concentrations is required in various fields, such as dairy industry, environmental protection, and clinical laboratories. The current...
-
2016-01-01
Song, Qiao, Gao, Yongfeng, Xu, Jiang-Fei, Qin, Bo, Serpe, Michael J., Zhang, Xi
This letter describes a new method for fabricating supramolecular microgels from supramonomers. To this end, we designed and assembled supramonomers with one acrylate moiety on each end on the basis of noncovalent host–guest interactions, which could be utilized as a cross-linker. Then...
-
2015
Berg, Darren, Mugo, Samuel M., Zhang, Qiang M., Serpe, Michael J.
Lipase-modified pH-responsive poly(N-isopropylacrylamide)-based microgels were synthesized. An optical device was subsequently fabricated by sandwiching the enzyme loaded responsive microgels between two thin Au layers, and their response to triolein, a model triglyceride, was investigated. The...
-
2015-01-01
Li, Xue, Gao, Yongfeng, Serpe, Michael J.
Poly (N‐isopropylacrylamide) (pNIPAm)‐based hydrogels and hydrogel particles (microgels) have been extensively studied since their discovery and “popularization” a few decades ago. While their uses seem to have no bounds, this Feature Article is focused on their development and application for...